Genome-based insights into the resistome and mobilome of multidrug-resistant Aeromonas sp. ARM81 isolated from wastewater
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- 作者：Marcin Adamczuk ; Lukasz Dziewit
- 关键词：Aeromonas sp. ARM81 ; Genome ; Antibiotic resistance gene ; Plasmid ; Transposable element
- 刊名：Archives of Microbiology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：199
- 期：1
- 页码：177-183
- 全文大小：668KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Microbiology; Microbial Ecology; Biochemistry, general; Cell Biology; Biotechnology; Ecology;
- 出版者：Springer Berlin Heidelberg
- ISSN：1432-072X
- 卷排序：199
文摘
The draft genome of multidrug-resistant Aeromonas sp. ARM81 isolated from a wastewater treatment plant in Warsaw (Poland) was obtained. Sequence analysis revealed multiple genes conferring resistance to aminoglycosides, β-lactams or tetracycline. Three different β-lactamase genes were identified, including an extended-spectrum β-lactamase gene blaPER-1. The antibiotic susceptibility was experimentally tested. Genome sequencing also allowed us to investigate the plasmidome and transposable mobilome of ARM81. Four plasmids, of which two carry phenotypic modules (i.e., genes encoding a zinc transporter ZitB and a putative glucosyltransferase), and 28 putative transposase genes were identified. The mobility of three insertion sequences (isoforms of previously identified elements ISAs12, ISKpn9 and ISAs26) was confirmed using trap plasmids.