Molecular characterization and genetic diversity of bovine Kobuvirus, Brazil
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- 作者：Marcelo Candido ; Maria Cryskely Agra Batinga ; Anna Luiza Farias Alencar…
- 关键词：Picornavirus ; Diarrhea ; Cattle diseases ; Molecular epidemiology
- 刊名：Virus Genes
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：53
- 期：1
- 页码：105-110
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Medical Microbiology; Virology; Plant Sciences;
- 出版者：Springer US
- ISSN：1572-994X
- 卷排序：53
文摘
It is suggested that Bovine kobuvirus (BKV) is involved in the etiology of gastroenteric diseases especially among calves; however, this association remains unknown. This study evaluated 216 fecal samples from cattle with and without diarrhea symptoms obtained from different regions of Brazil. A 216 bp fragment of the BKV 3D gene was amplified by RT-PCR in 14.4 % (31/216) of the studied samples, and 17 samples were subjected to nucleotide sequencing. All positive samples were obtained from animals aged less than 5 months, and most of animals presented diarrhea (p < 0.05). Phylogenetic analyses showed that the obtained sequences were grouped within the genogroup 2 of BKV forming subclades specific for each Brazilian municipality sampled. In addition, the alignment of the sequences revealed differences of nucleotides between sequences from different locations. Our results indicate for the first time that there is a regional genotypic differentiation of BKV in Brazil.