Phosphatidylinositol 3-kinase inhibitor(LY294002) induces apoptosis of human nasopharyngeal carcinoma in vitro and in vivo

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• 作者：Hanguo Jiang (1) (2)
  Desheng Fan (3)
  Gengyin Zhou (1)
  Xiaofang Li (3)
  Huihua Deng (3)
  
• 刊名：Journal of Experimental & Clinical Cancer Research
• 出版年：2010
• 出版时间：December 2010
• 年：2010
• 卷：29
• 期：1
• 全文大小：1450KB
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• 作者单位：Hanguo Jiang (1) (2)
  Desheng Fan (3)
  Gengyin Zhou (1)
  Xiaofang Li (3)
  Huihua Deng (3)
  
  1. Department of Pathology, School of Medicine, Shandong University, 250012, Jinan, China
  2. Department of Pathology, Guangdong Medical College, Zhanjiang, China
  3. Department of Pathology, Guangdong Medical College, 524023, Zhanjiang, China
  
• ISSN：1756-9966

文摘

Background To evaluate whether PI3K/Akt pathway could effect on apoptosis and its mechanism in nasopharyngeal carcinoma cells. Methods The activation of the PI3K/Akt and its effect on CNE-2Z cells in vivo and in vitro was investigated by MTT assay, flow cytometry, western blot, ELISA, terminal deoxyribonucleotide transferase-mediated nick-end labeling assays (TUNEL), and immunohistochemical analyses, using PI3K inhibitor, LY294002. Results The results showed that LY294002 inhibited the phosphorylating of Akt (S473), cell proliferation, and induced apoptosis in CNE-2Z cells. However, our experiment results also demonstrated that apoptosis-induced LY294002 was directly regulated by caspase-9 activation pathway. Conclusion These data suggested that PI3K inhibitor, LY294002, induced apoptosis by caspase-9 activation pathway and might be as a potentially useful target for therapeutic intervention in nasopharyngeal carcinoma patients.