Large-scale isolation of highly pure malaxinic acid from immature pear (Pyrus pyrifolia Nakai) fruit

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• 作者：Yu Geon Lee (1)
  Jeong-Yong Cho (1)
  Jun Park (1)
  Sang-Hyun Lee (2)
  Wol-Soo Kim (2) (3)
  Keun-Hyung Park (1)
  Jae-Hak Moon (1) (2)
  
• 关键词：pear ; Pyrus pyrifolia ; immature pear fruit ; malaxinic acid ; high purity
• 刊名：Food Science and Biotechnology
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：22
• 期：6
• 页码：1539-1545
• 全文大小：335 KB
• 作者单位：Yu Geon Lee (1)
  Jeong-Yong Cho (1)
  Jun Park (1)
  Sang-Hyun Lee (2)
  Wol-Soo Kim (2) (3)
  Keun-Hyung Park (1)
  Jae-Hak Moon (1) (2)
  
  1. Department of Food Science and Technology and Functional Food Research Center, Chonnam National University, Gwangju, 500-757, Korea
  2. Korea Pear Research Organization, Chonnam National University, Gwangju, 500-757, Korea
  3. Department of Horticulture, Chonnam National University, Gwangju, 500-757, Korea
  
• ISSN：2092-6456

文摘

Malaxinic acid [4-(O-β-d-glucopyranosyl)-3-(3-methyl-2-butenyl)benzoic acid; MA] is one of the main compounds contained in pear fruit. The antifungal, antibacterial, and anticancer effects of MA have been reported. However, additional evaluations on the biological activity of MA have not been fully performed due to the lack of an MA standard. Therefore, in the present study, we established a large-scale isolation method to obtain highly pure MA from immature pear (Pyrus pyrifolia Nakai) fruit. The EtOAc-soluble acidic fraction (134.75 g) of pear fruit (18 kg fresh wt.) 60% EtOH extract (1,081.9 g) was purified by Amberlite XAD-2 (H2O/EtOH, stepwise), Diaion HP-20 (H2O/MeOH, stepwise), and Sephadex LH-20 (80% MeOH) column chromatographies. Then, the fractions containing MA were finally purified by high performance liquid chromatography using an Amide RP-C16 column on semi-preparative scale. The isolated MA (793 mg) was >99% pure, and total recovery was estimated to be 61.7%.