Conserved and novel miRNAs in the legume Phaseolus vulgaris in response to stress

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• 作者：Catalina Arenas-Huertero ; Beatriz P¨¦rez ; Fernando Rabanal ; Daniel Blanco-Melo ; Carlos De la Rosa ; Georgina Estrada-Navarrete ; Federico Sanchez ; Alejandra Alicia Covarrubias and Jos¨¦ Luis Reyes
• 关键词：miRNAs ; Water deficit ; Glycine max ; Medicago truncatula ; Common bean ; Abscisic acid
• 刊名：Plant Molecular Biology
• 出版年：2009
• 出版时间：July, 2009
• 年：2009
• 卷：70
• 期：4
• 页码：385-401
• 全文大小：1.1 MB

文摘

MicroRNAs (miRNAs) are small RNA molecules recognized as important regulators of gene expression. Although plant miRNAs have been extensively studied in model systems, less is known in other plants with limited genome sequence data. We are interested in the identification of miRNAs in Phaseolus vulgaris (common bean) to uncover different plant strategies to cope with adverse conditions and because of its relevance as a crop in developing countries. Here we present the identification of conserved and candidate novel miRNAs in P. vulgaris present in different organs and growth conditions, including drought, abscisic acid treatment, and Rhizobium infection. We also identified cDNA sequences in public databases that represent the corresponding miRNA precursors. In addition, we predicted and validated target mRNAs amongst reported EST and cDNAs for P. vulgaris. We propose that the novel miRNAs present in common bean and other legumes, are involved in regulation of legume-specific processes including adaptation to diverse external cues.