Genetic Diversity and Characterization of a Core Collection of Malus Germplasm Using Simple Sequence Repeats (SSRs)

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• 作者：Sarah M. Potts (1)
  Yuepeng Han (2)
  M. Awais Khan (3)
  Mosbah M. Kushad (1)
  A. Lane Rayburn (1)
  Schuyler S. Korban (13) korban@illinois.edu
• 关键词：Apple &#8211 ; Microsatellite markers &#8211 ; Genetic diversity &#8211 ; Genetic relatedness &#8211 ; Germplasm characterization
• 刊名：Plant Molecular Biology Reporter
• 出版年：2012
• 出版时间：August 2012
• 年：2012
• 卷：30
• 期：4
• 页码：827-837
• 全文大小：377.1 KB
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• 作者单位：1. Department of Crop Sciences, University of Illinois at Urbana鈥揅hampaign, Urbana, IL 61801, USA2. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Moshan, Wuhan, 430074 People鈥檚 Republic of China3. Department of Natural Resources and Environmental Sciences, University of Illinois at Urbana鈥揅hampaign, Urbana, IL 61801, USA
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1572-9818

文摘

Simple sequence repeats (SSRs) were used to assess genetic diversity and study genetic relatedness in a large collection of Malus germplasm. A total of 164 accessions from the Malus core collection, maintained at the University of Illinois, were genotyped using apple SSR markers. Each of the accessions was genotyped using a single robust SSR marker from each of the 17 different linkage groups in Malus. Data were subjected to principal component analysis, and a dendrogram was constructed to establish genetic relatedness. As expected, this diverse core collection showed high allelic diversity; moreover, this allelic diversity was higher than that previously reported. Cluster analysis revealed the presence of four distinct clusters of accessions in this collection.