Variability in Mucuna pruriens L. germplasm for L-Dopa, an anti parkinsonian agent

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• 作者：Archana P. Raina (1) aprraina@yahoo.co.in
  J. B. Tomar (2)
  M. Dutta (1)
• 关键词：Germplasm &#8211 ; HPTLC &#8211 ; L ; Dopa &#8211 ; Mucuna pruriens &#8211 ; Parkinsonism
• 刊名：Genetic Resources and Crop Evolution
• 出版年：2012
• 出版时间：August 2012
• 年：2012
• 卷：59
• 期：6
• 页码：1207-1212
• 全文大小：1.1 MB
• 参考文献：1. Anonymous (1985) The Wealth of India. Raw materials. CSIR, New Delhi, vol 6, pp 442
  2. Bammi RK, Gangadhar Rao G (1982) Cultivation of Mucuna pruriens. In: Atal CK, Kapur BMK (eds) Cultivation and Utilization of Medicinal Plants. CSIR, Jammu-Tawi, pp 447–449
  3. Bell EA, Jansen DS (1971) Medical and ecological considerations of L-dopa and 5-HTP in seeds. Nature 229:136–137
  4. Brain KR (1976) Accumulation of L-dopa in cultures from Mucuna pruriens. Pl Sci Lett 7:157–161
  5. Daxenbichler ME, Vanetten CH, Hallinan FA (1971) Seeds as sources of L-dopa. J Med Chem 14:463–466
  6. Daxenbichler ME, Vanetten CH, Earle RE, Tallent WH (1972) L-Dopa recovery from Mucuna seed. J Agr Food Chem 20:1046–1048
  7. Farooqi AA, Khan MM, Sundhara MA (1999) Production technology of medicinal and aromatic crops. Natural Remedies Pvt. Ltd, Bangalore, pp 26–28
  8. Lorenzetti F, Maclsaac S, Arnason JT, Awang DVC, Buckles D (1998) The phytochemistry, toxicology and food potentials of velvet bean (Mucuna Adans. spp. Fabaceae). In: Buckles D, Eteka A, Osiname O, Galiba M, Galiba G (eds) Cover crops in West Africa: contributing to sustainable agriculture. IDRC, Ottawa, pp 67–84
  9. Mennickent S, Nail M, Vega M, Dediego M (2007) Quantitative determination of L-dopa in tablets by high performance thin layer chromatography. J Sep Sci 30:1893–1898
  10. Modi KP, Patel NM (2008) Estimation of L-dopa from Mucuna pruriens Linn. and formulations containing Mucuna pruriens by HPTLC method. Chem Pharm Bull 56:357–359
  11. Parikh KM, Doshi VJ, Sawant SV, Salunkhe VB (1989) Estimation of L-dopa from plant Mucuna pruriens and its formulation using HPLC. Indian Drugs 27:353–356
  12. Prakash D, Tewari SK (1999) Variation of L-dopa contents in Mucuna species. J Med Arom Plant Sci 21:343–346
  13. Rastogi RP, Mehrotra BN (1991) A compendium of Indian medicinal plants, vol I. Central Drug Research, Institute/Publication and information Directorate, Lucknow/New Delhi
  14. Sastry CST, Kavathekar YY (1990) Plants for reclamation of Wastelands. Publications and Information Directorate, New Delhi, pp 317–318
  15. Sathiyanarayanan L, Arulmozhi S (2007) Mucuna pruriens Linn.—a comprehensive review. Pharmacogn Rev 1:157–162
  16. Siddharaju P, Becker K (2001) Rapid reversed-phase performance liquid chromatographic method for the quantification of L-dopa, non-methylated and methylated tetrahydroisoquinoline compounds from Mucuna beans. Food Chem 72:389–394
  17. Singh BM, Srivastava VK, Kidwai MA, Gupta V, Gupta R (1995) Aloe, Psoralea and Mucuna. In: Chadha KL, Gupta R (eds) Advances in horticulture. Medicinal and aromatic plants, vol 11. Malhotra Publ House, New Delhi, pp 515–525
  18. Singh AK, Malik SS, Tomar YS (2008) Studies on medicinal compound L-dopa in Mucuna pruriens Bak. Ind J Plant Genet Resour 21:217–220
• 作者单位：1. Germplasm Evaluation Division, National Bureau of Plant Genetic Resources, New Delhi, 110012 India2. National Bureau of Plant Genetic Resources Regional Station, Ranchi, India
• ISSN：1573-5109

文摘

Mucuna pruriens L. (Fabaceae) is a popular Indian medicinal plant, which has long been used in traditional Ayurvedic system of Indian medicine for diseases including parkinsonism. The seeds of M. pruriens contain 3,4-dihydroxyphenyl-L-alanine (L-dopa) as a major constituent, an intermediate precursor of the neurotransmitter dopamine. L-dopa extracted from seeds of Mucuna is more effective as drug than the synthetic counterpart. The plant is widely distributed throughout the India and in other parts of the tropics including Central and South America. The present study was aimed to screen thirty-eight accessions of Mucuna germplasm collected from different geographical regions of India for L-dopa content, an effective drug used for Parkinson’s disease. The L-dopa content in seeds of M. pruriens was quantitatively analyzed by high performance thin-layer chromatography (HPTLC) at 280 nm wavelength. Results indicated wide variability among thirty-eight accessions of M. pruriens for seed characters like seed colour, size and shape. The L-dopa content in seeds of M. pruriens ranged between 3.29 and 5.44 % (on dry weight basis) among different germplasm collections augmented from various parts of India. Promising accessions identified having high L-dopa content were IC551549 (5.44 %), IC551514 (5.41 %), IC331536 (5.40 %), IC552857 (5.36 %), IC385844 (5.32 %) and IC439197 (5.32 %). Due to the economic importance of the drug, these promising M. pruriens accessions can be exploited for large scale cultivation for their pharmacological important constituents mainly L-dopa content.