Hydrolysis of [17,17-^2H_2]gibberellinA_20-glucoside and[17,17-^2H_2]gibberellin A_20-glucosylester by Azospirillum lipofe

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• 作者：Piccoli ; Patricia ; Lucangeli ; Carlos D. ; Schneider ; Gernot ; Bottini ; Rubé ; n ; Schneider ; Gernot
• 关键词：Azospirillum lipoferum ; conjugates ; GAs ; hydrolase activity
• 刊名：Plant Growth Regulation
• 出版年：1997
• 出版时间：1997
• 年：1997
• 卷：23
• 期：3
• 页码：179-182
• 全文大小：55 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5087

文摘

Azospirillum lipoferum strain USA 5b, a gibberellin producing bacterium,was cultured in a nitrogen-free biotin-based chemically-defined medium inthe presence of the glucosyl ester or the 13-O-glucoside of [17,17-$^2$H$\_2$]-gibberellin A$\_20$. The[17,17-$^2$H$\_2$]-gibberellin A$\_20$conjugates were added at both the stationary phase of the cultures and atthe beginning of the growth curve. Metabolism of the conjugates was examinedafter 72 h of incubation using capillary gas chromatography-massspectrometry, with identification by full scan mass spectra. Metabolitesidentified were [17,17-$^2$H$\_2$]-gibberellinA$\_20$, [17,17-$^2$H$\_2$]-gibberellinA$\_1$ and [17,17-$^2$H$\_2$]-gibberellinA$\_3$. Also, in the Azospirillum cultures fed at the beginning ofthe growth curve, gibberellin A$\_5$ and gibberellinA$\_20$ were characterized as endogenous by mass spectrometry/fullspectrum. These results support the concept that the growth promotion inplants that is induced by Azospirillum infection may occur by a combinationof both gibberellin production and gibberellin-glucoside/glucosyl esterdeconjugation by the bacterium.