Genetic characterization of Fasciola gigantica from different geographical regions of India by ribosomal DNA markers
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- 作者：O. K. Raina ; Siju Susan Jacob ; M. Sankar ; D. Bhattacharya…
- 关键词：Fasciola gigantica ; 28S ribosomal DNA ; ITS ; 2 ; Genetic characterization
- 刊名：Journal of Parasitic Diseases
- 出版年：2015
- 出版时间：March 2015
- 年：2015
- 卷：39
- 期：1
- 页码：27-32
- 全文大小：431 KB
- 参考文献：1. Agatsuma T, Arakawa Y, Iwagami M, Honzako Y, Cahyaningsih U, Kang SY, Hong SJ (2000) Molecular evidence of natural hybridization between / Fasciola hepatica and / F. gigantica. Parasitol Int 49:231-38 f="http://dx.doi.org/10.1016/S1383-5769(00)00051-9" target="_blank" title="It opens in new window">CrossRef
2. Ali H, Ai L, Song HQ, Ali S, Lin RQ, Seyni B, Issa G, Zhu XQ (2008) Genetic characterization of / Fasciola samples from different host species and geographical localities revealed the existence of / F. hepatica and / F. gigantica in Niger. Parasitol Res 102:1021-024 f="http://dx.doi.org/10.1007/s00436-007-0870-7" target="_blank" title="It opens in new window">CrossRef
3. Ashrafi K, Valero MA, Panova M, Periago MV, Massoud J, Mas-Coma S (2006) Phenotypic analysis of adults of / Fasciola hepatica, / Fasciola gigantica and intermediate forms from the endemic region of Gilan, Iran. Parasitol Int 55:249-60 f="http://dx.doi.org/10.1016/j.parint.2006.06.003" target="_blank" title="It opens in new window">CrossRef
4. Douady CJ, Delsuc F, Boucher Y, Doolittle WF, Douzer EJ (2003) Comparison of Bayesian and maximum likelihood bootstrap measures of phylogenetic reliability. Mol Biol Evol 20:248-54 f="http://dx.doi.org/10.1093/molbev/msg042" target="_blank" title="It opens in new window">CrossRef
5. Gunasekar KR, Tewari AK, Sreekumar C, Gupta SC, Rao JR (2008) Elucidation of genetic variability among different isolates of / Fasciola gigantica (giant liver fluke) using random-amplified polymorphic DNA-polymerase chain reaction. Parasitol Res 103:1075-081 f="http://dx.doi.org/10.1007/s00436-008-1095-0" target="_blank" title="It opens in new window">CrossRef
6. Huang WY, He B, Wang CR, Zhu XQ (2004) Characterization of / Fasciola species from Mainland China by ITS-2 ribosomal DNA sequence. Vet Parasitol 120:75-3 f="http://dx.doi.org/10.1016/j.vetpar.2003.12.006" target="_blank" title="It opens in new window">CrossRef
7. Hurtrez-Bousses S, Durand P, Jabbour-Zahab R, Guegan JF, Meunier C, Bargues MD, Mas-Coma S, Renaud F (2004) Isolation and characterization of microsatellite markers in the liver fluke ( / Fasciola hepatica). Mol Ecol Notes 4:689-90 f="http://dx.doi.org/10.1111/j.1471-8286.2004.00786.x" target="_blank" title="It opens in new window">CrossRef
8. Itagaki T, Kikawa M, Terasaki K, Shibahara T, Fukuda K (2005a) Molecular characterization of parthenogenic / Fasciola sp. in Korea on the basis of DNA sequences of ribosomal ITS1 and mitochondrial NDI gene. J Med Vet Sci 67:1115-118 f="http://dx.doi.org/10.1292/jvms.67.1115" target="_blank" title="It opens in new window">CrossRef
9. Itagaki T, Kikawa M, Sakaguchi K, Shimo J, Terasaki K, Shibahara T, Fukuda K (2005b) Genetic characterization of parthenogenic / Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA. Parasitology 131:679-85 f="http://dx.doi.org/10.1017/S0031182005008292" target="_blank" title="It opens in new window">CrossRef
10. Itagaki T, Sakaguchi K, Terasaki K, Sasaki O, Yoshihara S, Van Dung T (2009) Occurrence of spermic diploid and aspermic triploid forms of / Fasciola in Vietnam and their molecular characterization based on nuclear and mitochondrial DNA. Parasitol Int 58:81-5 f="http://dx.doi.org/10.1016/j.parint.2008.11.003" target="_blank" title="It opens in new window">CrossRef
11. Le TH, De NV, Agatsuma T, Nguyen TGT, Nguyen QD, McManus DP, Blair D (2008) Human fascioliasis and the presence of hybrid/introgressed forms of / Fasciola in Vietnam. Int J Parasitol 38:725-30 f="http://dx.doi.org/10.1016/j.ijpara.2007.10.003" target="_blank" title="It opens in new window">CrossRef
12. Lin RQ, Dong SJ, Nie K, Wang CR, Song HQ, Li AX, Huang WY, Zhu XQ (2007) Sequence analysis of the first internal transcribed spacer of rDNA supports the existence of the intermediate / Fasciola between / F. hepatica and / F. / gigantica in mainland China. Parasitol Res 101:813-17 f="http://dx.doi.org/10.1007/s00436-007-0512-0" target="_blank" title="It opens in new window">CrossRef
13. Marcilla A, Bargues MD, Mas-Coma S (2002) A PCR-RFLP assay for the distinction between / Fasciola hepatica and / Fasciola gigantica. Mol Cel Probes 16:327-33 f="http://dx.doi.org/10.1006/mcpr.2002.0429" target="_blank" title="It opens in new window">CrossRef
14. Peng M, Ichinomiya M, Ohtori M, Ichikawa M, Shibahara T, Itagaki T (2009) Molecular characterization of / Fasciola hepatica, / Fasciola gigantica, and aspermic / Fasciola sp. in China based on nuclear and mitochondrial DNA. Parasitol Res 105:809-15 fectious Diseases; Health Promotion and Disease Prevention; Medicine/Public Health, general; - 出版者：Springer India
- ISSN：0975-0703
文摘
Ribosomal DNA sequences of the second internal transcribed spacer (ITS-2) and 28S ribosomal DNA (618?bp) of Fasciola gigantica collected from cattle and buffaloes from four different geographical locations of India, were characterized for genotyping. ITS-2 sequence was analyzed in 28 worms that was typical of F. gigantica and differed at six positions, with one of these being a distinguishing deletion (T) at the 327th position in F. gigantica relative to F. hepatica. However, Fasciola specimens also showed intraspecies sequence polymorphism in the ITS-2, with two different ITS-2 sequences existing in the ribosomal DNA (rDNA) array within a single Fasciola worm. One of the sequences was identical to that of F. gigantica and the other showed extensive sequence polymorphism in the ITS-2. Using BspH1-restriction fragment length polymorphism, six variable ITS-2 sequences in F. gigantica were identified within these parasite specimens and were found distributed in these four geographical regions. 28S rDNA sequence of 24 flukes, collected from the above four geographical regions, showed a single nucleotide polymorphism at 284th nucleotide (G/A). Analyzing the sequence data of 28S rDNA of F. gigantica available from some African and Asian countries for this polymorphic 284th nucleotide position, it is proposed that there are two basic lineages of the F. gigantica for 28S rDNA existing in the fluke populations from five African and several Asian countries.