Significance of a common 65?kDa antigen in the experimental fasciolosis and toxoplasmosis

详细信息    查看全文

• 作者：Raafat Mohamed Shaapan ; Nagwa Ibrahim Toaleb…
• 关键词：Cross reactive antigens ; F. gigantica ; T. gondii ; ELISA ; SDS ; PAGE ; Western blot
• 刊名：Journal of Parasitic Diseases
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：39
• 期：3
• 页码：550-556
• 全文大小：760 KB
• 参考文献：Abdel-Rahman EH, Abdel-Megeed KN (2000) Molecular identity of major cross-reactive adult antigen in Fasciola gigantica; Toxocara vitulorum and Moniezia expansa. J Egypt Soc Parasitol 30:561-71PubMed
  Abdel-Rahman EH, Abdel-Megeed KN, Abuel-Ezz NMT (2003) Cross-reaction; a common trait among helminthes. J Egypt Soc Parasitol 33:457-71PubMed
  Anuracpreeda P, Chawengkirtikulc R, Tinikulb Y, Poljaroenb J, Chotwiwatthanakun C, Sobhonb P (2013) Diagnosis of Fasciola gigantica infection using a monoclonal antibody-based sandwich ELISA for detection of circulating cathepsin B3 protease. Acta Trop 127:38-5CrossRef PubMed
  Boukli NM, Delgado B, Ricaurte M, Espino AM (2011) Fasciola hepatica and Schistosoma mansoni: identification of common proteins by comparative proteomic analysis. J Parasitol 97:852-61PubMed Central CrossRef PubMed
  Delibas SB, Ertabaklar H, Ertug S (2006) Evaluation of antigenic variations between two virulent toxoplasma strains. J Med Microbiol 55:1333-335CrossRef PubMed
  Dubey JP, Beattie CP (1988) Toxoplasmosis of animals and man. CRC Press, Boca Raton, pp 1-20
  Dubey JP, Ferreira LR, Martins J, McLeod R (2012) Oral oocyst-induced mouse model of toxoplasmosis: effect of infection with Toxoplasma gondii strains of different genotypes, dose, and mouse strains (transgenic, out
  ed, in
  ed) on pathogenesis and mortality. Parasitology 139(1):1-3PubMed Central CrossRef PubMed
  El-Moghazy FM, Abdel-Rahman EH (2012) Cross-reaction as a common phenomenon among tissue parasites in farm animals. Glob Vet 8:367-73
  Espindola NM, De Gaspari EN, Nakamura PM, Vaz AJ (2000) Cross-reaction of anti-Taenia crassiceps cysticerci immune antibodies with Taenia solium antigen. Vet Parasitol 89:321-26CrossRef PubMed
  Ferrer E, Benitez L, Foster-Cuevas M, Bryce D, Wamae LW, Onyango-Abuje JA, Garate T, Harrison LJ, Parkhouses RM (2003) Taenia saginata derived synthetic peptides with potential for the diagnosis of bovine cysticercosis. Vet Parasitol 111:83-4CrossRef PubMed
  Gamble HR, Dubey JP, Lambillotte DN (2005) Comparison of a commercial ELISA with the modified agglutination test for detection of Toxoplasma infection in the domestic pig. Vet Parasitol 128:177-81CrossRef PubMed
  Hassan SE, Shaapan RM, Ghazy AA, Abdel-Rahman EH (2012) Evaluation of purified horse derived antigen in the diagnosis of human toxoplasmosis. Glob Vet 9:630-34
  Hassanain MA, Hassanain NA, Shaapan RM, El Fadaly HA, El-Moghazy FM (2009) Problem of toxoplasmosis and detection of possible cross-reactivity with Trichinella spiralis. Proceeding of the XIV ISAH congress, 19-3 July, Vechta, Germany, pp: 97-00
  Hassanain MA, El Fadaly HA, Shaapan RM, Hassanain NA, Barakat AM (2011) Biological assay of Toxoplasma gondii Egyptian mutton isolates. Int J Z Res 7:330-37CrossRef
  Hillyer GM, Soler De Galanes M, Rodriguez-Perez J, Bjorland J, De Lagrava MS, Guzman SR, Bryan RT (1992) Use of the falcon assay screening test-enzyme-linked immunosorbent assay (FAST-ELISA) and the enzyme-linked immunoelectro-transfer blot (EITB) to determine the prevalence of human fascioliasis in the Bolivian Altiplano. Am J Trop Med Hyg 46:603-09PubMed
  Howe DK, Sibley LD (1995) Toxoplasma gondii comprises three clonal lineages: correlation of parasite genotype with human disease. J Infect Dis 172:1561-566CrossRef PubMed
  Ishida G, Rubinsky-Elefant G, Ferreira AW, Hoshino-Shimizu S, Vaz AJ (2003) Helminth antigens (Taenia solium, Taenia crassiceps, Toxocara canis, Schistosoma mansoni and Echinococcus granulosus) and cross-reactivities in human infections and immunized animals. Acta Trop 89:73-4CrossRef PubMed
  Johnson MA, McDonald PJ, Neoh HS (1979) Kinetics of the growth of Toxoplasma gondii (RH strain) in mice. Int J Parasitol 9:55-6CrossRef PubMed
  Kandil OM, Mahmoud MS, El-Moghazy FM (2005) Comparative characterization and DNA analysis of hydatid and Cysticercus tenuicollis cyst fluids. Vet Med J 53:127-42
  Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-85CrossRef PubMed
  Lind P, Haugegaard J, Wingstrand A, Henrisken SA (1997) The time course of the specific antibody response by various ELISAs in pigs experimentally infected with Toxoplasma gondii. Vet Parasitol 71:1-5CrossRef PubMed
  Losada S, Chacon N, Colmenares C, Bermudez H, Lorenzo A, Pointier JP, Theron A, Alarcon de Noya B, Noya O (2005) Schistosoma cross-reactivity and antigenic community among different species. Exp Parasitol 111:182-90CrossRef PubMed
  Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:262-75
  Miller CMD, Smith NC, Ikin RJ, Boulter NR, Dalton JP (2009) Immunological interactions between 2 common pathogens, Th1-inducing protozoan Toxoplasma gondii and the Th2-inducing helminth Fasciola hepatica. PLoS ONE 4(5):1-0CrossRef
  Muro A, Ramajo V, López J, Simón F, Hillyer
• 作者单位：Raafat Mohamed Shaapan (1)
  Nagwa Ibrahim Toaleb (2)
  Eman Hussein Abdel-Rahman (2)
  
  1. Department of Zoonosis, Veterinary Research Division, National Research Center, Post Box 12622, El-Tahrir Street, Dokki, Giza, Egypt
  2. Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Center, Giza, Egypt
  
• 刊物主题：Infectious Diseases; Health Promotion and Disease Prevention; Medicine/Public Health, general;
• 出版者：Springer India
• ISSN：0975-0703

文摘

In the current study, cross-reaction between two important zoonotic parasites; extracellular helminthes Fasciola gigantica and intracellular protozoa Toxoplasma gondii was proved by enzyme linked immunosorbent assay. Five antigens were used to identify and compare the cross-binding activities in the prepared antisera. Two F. gigantica antigens; adult flukes (FgA) and eggs (FgEA) were used to detect IgG in T. gondii naturally infected human sera (TgIHS) and experimentally infected sera of sheep (TgISS), mice (TgIMS) and rats (TgIRS). Three types of T. gondii antigens; RH (TgRHA), local sheep isolate (TgLA) and ME49 isolate (TgMEA) were used to detect cross binding activities in F. gigantica experimental infected rabbit sera (FgIRS) and F. gigantica naturally infected bovine sera (FgIBS). The cross-binding activities in the prepared antisera were strongly directed towards FgA and TgLA rather than the other antigens. The characterization of the five antigens using SDS-PAGE showed 4 common bands of FgA and TgLA; 165, 97, 76, and 65 kDa. While two common bands were observed between TgRHA, TgMEA and FgA; 165, and 65 kDa. Whereas, two common bands found between three types of T. gondii antigens and FgEA were identified; 165 and 65 kDa. The immunogenic cross-reactive bands between FgA and TgLA with F. gigantica infected bovine sera were identified by immunoblot. In FgA, the common immunogenic bands were 165, 65 and 14 kDa. While in TgLA, common immunogenic bands were 165 and 65 kDa. Whereas, the common immunogenic band between FgA and TgLA identified with T. gondii experimentally infected sheep sera was 65 kDa. The current research proves cross reaction between F. gigantica and T. gondii. One common band of 65 kDa showed broad immunogenic cross-reactivity with the developed antisera raising the prospect of being putative common immunodiagnostic candidate of both infections. Keywords Cross reactive antigens F. gigantica T. gondii ELISA SDS-PAGE Western blot