An enzyme-linked immunosorbent assay for diagnosis of Fasciola gigantica infection in cattle and buffaloes

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• 作者：C. M. Krishna Murthy ; Placid E. D. Souza
• 关键词：Fasciola gigantica ; Excretory–secretory antigen ; Sensitivity
• 刊名：Journal of Parasitic Diseases
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：39
• 期：4
• 页码：783-785
• 全文大小：333 KB
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• 作者单位：C. M. Krishna Murthy (1)
  Placid E. D. Souza (2)
  
  1. Department of Veterinary Parasitology, Veterinary College, Vinobanagar, Shimoga, Karnataka, 577204, India
  2. Centre of Advanced Faculty Training, Deartment of Veterinary Parasitology, Veterinary College, Hebbal, KVAFSU, Bangalore, 560024, India
  
• 刊物主题：Infectious Diseases; Health Promotion and Disease Prevention; Medicine/Public Health, general;
• 出版者：Springer India
• ISSN：0975-0703

文摘

The enzyme-linked immunosorbent assay (ELISA) was evaluated for the diagnosis of Fasciola gigantica infection in cattle and buffaloes. The excretory–secretory (E–S Ag) antigen of F. gigantica adult flukes obtained after invitro incubation was used as an antigen. The test was conducted with 276 sera collected from cattle and buffaloes which included 22 sera each from naturally infected cattle and buffaloes (known positive serum) and with similar number of samples with healthy cattle and buffaloes (known negative serum). The positive results were observed in 18 and 19 of the sera from naturally infected cattle and buffaloes with sensitivity of 81.8 and 86.3 % respectively. Out of 188 serum samples which were found negative on faecal examination 32 (34 %) sera of cattle and 40 (42.5 %) sera of buffaloes were found positive by ELISA respectively. The sensitivity of the test was found to be 91.6 and 95.6 % in cattle and buffaloes respectively. Keywords Fasciola gigantica Excretory–secretory antigen Sensitivity