2D-PAGE analysis of the soluble proteins of the tropical liver fluke, Fasciola gigantica and biliary amphistome, Gigantocotyle explanatum, concurrently infecting Bubalus bubalis
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- 作者：Yasir A. Khan ; Mohd Abdul Hannan Khan ; S. M. A. Abidi
- 刊名：Journal of Parasitic Diseases
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：40
- 期：3
- 页码：910-913
- 全文大小：595 KB
- 刊物主题：Infectious Diseases; Health Promotion and Disease Prevention; Medicine/Public Health, general;
- 出版者：Springer India
- ISSN：0975-0703
- 卷排序：40
文摘
The digenetic trematodes, Fasciola gigantica and Gigantocotyle explanatum, belonging to the family Fasciolidae and Paramphistomidae respectively, have been often found to concurrently infect the liver of Indian water buffalo Bubalus bubalis, causing serious pathological damage to the vital organ, incurring huge economic losses. In the present study the soluble gene products of both F. gigantica and G. explanatum were analyzed by 2 dimensional polyacrylamide gel electrophoresis. The soluble proteomic profile revealed considerable similarity as well as differences in the size, distribution pattern, total number, the isoelectric point (pI) and molecular weight (Mr) of the resolved polypeptide spots. The maximum number of polypeptide spots with a molecular weight range of >10 to 160 kDa were recorded with a pI range of 7–9 followed by pI range of 5–7, 9–10 and 3–5 in both the parasites. However, considerable variation was recorded in the Mr of the polypeptides belonging to each pI range. The genetic heterogeneity could be an obvious contributing factor for such differences but some polypeptides appeared to be conserved in the two species. The molecular similarities and the habitat preference by these worms may be a consequence of microenvironmental cues that guide these flukes to reach their habitat through different routes and establish a successful host-parasite relationship.KeywordsDigenetic trematodes2D PAGEConcurrent infectionBubalus bubalis