Evaluation of novel beta-lactam antibiotics in comparison to cefotaxime on plant regeneration of Citrus sinensis L. Osb.
详细信息 查看全文
- 作者：Amanda Ferreira da Silva Mendes ; Luciana Cardoso Cidade ; Maria Luiza Peixoto de Oliveira ; Wagner Campos Otoni ; Walter Dos Santos Soares-Filho and Marcio Gilberto Cardoso Costa
- 关键词：Agrobacterium tumefaciens ; Bactericidal activity ; Phytotoxicity ; Morphogenesis
- 刊名：Plant Cell, Tissue and Organ Culture
- 出版年：2009
- 出版时间：June, 2009
- 年：2009
- 卷：97
- 期：3
- 页码：331-336
- 全文大小：285.9 KB
文摘
Identification of beta-lactam antibiotics that have negligible effects on plant regeneration is a critical step towards the establishment of a reliable Agrobacterium-mediated transformation protocol for perennial trees. In the present report, we have evaluated the effects of the novel beta-lactam antibiotics meropenem and timentin on plant regeneration of a perennial woody fruit plant, Citrus sinensis, in comparison with the commonly used beta-lactam cefotaxime. It was observed that, in contrast to cefotaxime, meropenem and timentin had a positive or no detrimental effect on the shoot regeneration from epicotyl explants. Residual effects of the beta-lactams from shoot regeneration medium also affected the subsequent ability of the roots to elongate. The addition of meropenem and/or timentin in the rooting medium mostly improved or did not affect the rooting ability of the adventitious shoots. These data indicated that meropenem and timentin can positively replace cefotaxime in Agrobacterium-mediated transformation of C. sinensis.