Changes in expression of Class 3 Semaphorins and their receptors during development of the rat retina and superior colliculus
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- 作者：Anil Sharma (1)
Chrisna J LeVaillant (1)
Giles W Plant (1) (2)
Alan R Harvey (1)
1. School of Anatomy ; Physiology and Human Biology ; The University of Western Australia ; 35 Stirling Highway ; Crawley ; WA ; 6009 ; Australia
2. Stanford Partnership for Spinal Cord Injury and Repair ; Department of Neurosurgery ; School of Medicine ; Stanford University ; Lorry I. Lokey Stem Cell Research Building (SIM1) ; 265 Campus Drive ; Stanford ; CA ; 94305-5454 ; USA - 关键词：Retinal ganglion cells ; Collapse assay ; qPCR ; Neuropilins ; Plexins ; Cell adhesion molecules
- 刊名：BMC Developmental Biology
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：14
- 期：1
- 全文大小：1,456 KB
- 参考文献：1. Scicolone, G, Ortalli, AL, Carri, NG (2009) Key roles of Ephs and ephrins in retinotectal topographic map formation. Brain Res Bull 79: pp. 227-247
2. Erskine, L, Herrera, E (2007) The retinal ganglion cell axon鈥檚 journey: insights into molecular mechanisms of axon guidance. Dev Biol 308: pp. 1-14
3. Isenmann, S, Kretz, A, Cellerino, A (2003) Molecular determinants of retinal ganglion cell development, survival, and regeneration. Prog Retin Eye Res 22: pp. 483-543
4. Feldheim, DA, O鈥橪eary, DDM (2010) Visual map development: bidirectional signaling, bifunctional guidance molecules, and competition. Cold Spring Harb Perspect Biol 2: pp. a001768
5. McLaughlin, T, Hindges, R, O鈥橪eary, DD (2003) Regulation of axial patterning of the retina and its topographic mapping in the brain. Curr Opin Neurobiol 13: pp. 57-69
6. Reese, BE (2011) Development of the retina and optic pathway. Vis Res 51: pp. 613-632
7. Harada, T, Harada, C, Parada, LF (2007) Molecular regulation of visual system development: more than meets the eye. Genes Dev 21: pp. 367-378
8. Campbell, DS, Regan, AG, Lopez, JS, Tannahill, D, Harris, WA, Holt, CE (2001) Semaphorin 3A elicits stage-dependent collapse, turning, and branching in Xenopus retinal growth cones. J Neurosci 21: pp. 8538-8547
9. Kita, EM, Bertolesi, GE, Hehr, CL, Johnston, J, McFarlane, S (2013) Neuropilin-1 biases dendrite polarization in the retina. Development 140: pp. 2933-2941
10. Halloran, MC, Severance, SM, Yee, CS, Gemza, DL, Raper, JA, Kuwada, JY (1999) Analysis of a Zebrafish semaphorin reveals potential functions in vivo. Dev Dyn 214: pp. 13-25
11. Liu, Y, Berndt, J, Su, F, Tawarayama, H, Shoji, W, Kuwada, JY, Halloran, MC (2004) Semaphorin3D guides retinal axons along the dorsoventral axis of the tectum. J Neurosci 24: pp. 310-318
12. Sakai, JA, Halloran, MC (2006) Semaphorin 3d guides laterality of retinal ganglion cell projections in zebrafish. Development 133: pp. 1035-1044
13. Callander, DC, Lamont, RE, Childs, SJ, McFarlane, S (2007) Expression of multiple class three semaphorins in the retina and along the path of zebrafish retinal axons. Dev Dyn 236: pp. 2918-2924
14. Dell, AL, Fried-Cassorla, E, Xu, H, Raper, JA (2013) cAMP-induced expression of neuropilin1 promotes retinal axon crossing in the zebrafish optic chiasm. J Neurosci 33: pp. 11076-11088
15. Rosenzweig, S, Raz-Prag, D, Nitzan, A, Galron, R, Paz, M, Jeserich, G, Neufeld, G, Barzilai, A, Solomon, AS (2010) Sema-3A indirectly disrupts the regeneration process of goldfish optic nerve after controlled injury. Graefes Arch Clin Exp Ophthalmol 248: pp. 1423-1435
16. Luo, Y, Shepherd, I, Li, J, Renzi, MJ, Chang, S, Raper, JA (1995) A family of molecules related to collapsin in the embryonic chick nervous system. Neuron 14: pp. 1131
17. Shepherd, I, Luo, Y, Raper, JA, Chang, S (1996) The distribution of collapsin-1 mRNA in the developing chick nervous system. Dev Biol 173: pp. 185-199
18. Takahashi, T, Nakamura, F, Jin, Z, Kalb, RG, Strittmatter, SM (1998) Semaphorins A and E act as antagonists of neuropilin-1 and agonists of neuropilin-2 receptors. Nat Neurosci 1: pp. 487-493
19. Henke-Fahle, S, Beck, KW, P眉schel, AW (2001) Differential responsiveness to the chemorepellent Semaphorin 3A distinguishes Ipsi- and contralaterally projecting axons in the chick midbrain. Dev Biol 237: pp. 381-397
20. Steinbach, K, Volkmer, H, Schlosshauer, B (2002) Semaphorin 3E/collapsin-5 inhibits growing retinal axons. Exp Cell Res 279: pp. 52-61
21. Steffensky, M, Steinbach, K, Schwarz, U, Schlosshauer, B (2006) Differential impact of semaphorin 3E and 3A on CNS axons. Int J Dev Neurosci 24: pp. 65-72
22. Matsuoka, RL, Chivatakarn, O, Badea, TC, Samuels, IS, Cahill, H, Katayama, K, Kumar, SR, Suto, F, Chedotal, A, Peachey, NS, Nathans, J, Yoshida, Y, Giger, RJ, Kolodkin, AL (2011) Class 5 transmembrane semaphorins control selective Mammalian retinal lamination and function. Neuron 71: pp. 460-473
23. Matsuoka, RL, Nguyen-Ba-Charvet, KT, Parray, A, Badea, TC, Chedotal, A, Kolodkin, AL (2011) Transmembrane semaphorin signalling controls laminar stratification in the mammalian retina. Nature 470: pp. 259-263
24. Sun, LO, Jiang, Z, Rivlin-Etzion, M, Hand, R, Brady, CM, Matsuoka, RL, Yau, KW, Feller, MB, Kolodkin, AL (2013) On and off retinal circuit assembly by divergent molecular mechanisms. Science 342: pp. 1241974
25. Kuwajima, T, Yoshida, Y, Takegahara, N, Petros, TJ, Kumanogoh, A, Jessell, TM, Sakurai, T, Mason, C (2012) Optic chiasm presentation of Semaphorin6D in the context of Plexin-A1 and Nr-CAM promotes retinal axon midline crossing. Neuron 74: pp. 676-690
Unified Nomenclature for the Semaphorins/Collapsins. Cell 97: pp. 551-552
26. Winter, F, Cui, Q, Symons, N, Verhaagen, J, Harvey, AR (2004) Expression of class-3 semaphorins and their receptors in the neonatal and adult rat retina. Invest Ophthalmol Vis Sci 45: pp. 4554-4562
27. Roche, J, Boldog, F, Robinson, M, Robinson, L, Varella-Garcia, M, Swanton, M, Waggoner, B, Fishel, R, Franklin, W, Gemmill, R, Drabkin, H (1996) Distinct 3p21.3 deletions in lung cancer and identification of a new human semaphorin. Oncogene 12: pp. 1289-1297
28. Kolodkin, AL, Matthes, DJ, Goodman, CS (1993) The semaphorin genes encode a family of transmembrane and secreted growth cone guidance molecules. Cell 75: pp. 1389
29. Luo, Y, Raible, D, Raper, JA (1993) Collapsin: a protein in brain that induces the collapse and paralysis of neuronal growth cones. Cell 75: pp. 217
30. Feiner, L, Koppel, AM, Kobayashi, H, Raper, JA (1997) Secreted chick semaphorins bind recombinant neuropilin with similar affinities but bind different subsets of neurons in situ. Neuron 19: pp. 539-545
31. P眉schel, AW, Adams, RH, Betz, H (1995) Murine semaphorin D/collapsin is a member of a diverse gene family and creates domains inhibitory for axonal extension. Neuron 14: pp. 941
32. Sekido, Y, Bader, S, Latif, F, Chen, J-Y, Duh, F-M, Wei, M-H, Albanesi, JP, Lee, C-C, Lerman, MI, Minna, JD (1996) Human semaphorins A (V) and IV reside in the 3p21.3 small cell lung cancer deletion region and demonstrate distinct expression patterns. Proc Natl Acad Sci U S A 93: pp. 4120-4125
33. Xiang, R (1996) Isolation of the human semaphorin III/F gene (SEMA3F) at chromosome 3p21, a region deleted in lung cancer. Genomics 32: pp. 39
34. Taniguchi, M, Masuda, T, Fukaya, M, Kataoka, H, Mishina, M, Yaginuma, H, Watanabe, M, Shimizu, T (2005) Identification and characterization of a novel member of murine semaphorin family. Genes Cells 10: pp. 785-792
35. Stevens, CB, Halloran, MC (2005) Developmental expression of sema3G, a novel zebrafish semaphorin. Gene Expr Patterns 5: pp. 647-653
36. Sharma, A, Verhaagen, J, Harvey, AR (2012) Receptor complexes for each of the Class 3 Semaphorins. Front Cell Neurosci 6: pp. 28
37. Kolodkin, AL, Matthes, DJ, O鈥機onnor, TP, Patel, NH, Admon, A, Bentley, D, Goodman, CS (1992) Fasciclin IV: sequence, expression, and function during growth cone guidance in the grasshopper embryo. Neuron 9: pp. 831-845
38. Roth, L, Koncina, E, Satkauskas, S, Cremel, G, Aunis, D, Bagnard, D (2009) The many faces of semaphorins: from development to pathology. Cell Mol Life Sci 66: pp. 649-666
39. Yazdani, U, Terman, JR (2006) The semaphorins. Genome Biol 7: pp. 211
40. Mizui, M, Kumanogoh, A, Kikutani, H (2009) Immune semaphorins: novel features of neural guidance molecules. J Clin Immunol 29: pp. 1-11
41. Pasterkamp, RJ, Giger, RJ (2009) Semaphorin function in neural plasticity and disease. Curr Opin Neurobiol 19: pp. 263-274
42. Suzuki, K, Kumanogoh, A, Kikutani, H (2008) Semaphorins and their receptors in immune cell interactions. Nat Immunol 9: pp. 17-23
43. Neufeld, G, Kessler, O (2008) The semaphorins: versatile regulators of tumour progression and tumour angiogenesis. Nat Rev Cancer 8: pp. 632-645
44. Claudepierre, T, Koncina, E, Pfrieger, FW, Bagnard, D, Aunis, D, Reber, M (2008) Implication of neuropilin 2/semaphorin 3F in retinocollicular map formation. Dev Dyn 237: pp. 3394-3403
45. Kim, J, Oh, WJ, Gaiano, N, Yoshida, Y, Gu, C (2011) Semaphorin 3E-Plexin-D1 signaling regulates VEGF function in developmental angiogenesis via a feedback mechanism. Genes Dev 25: pp. 1399-1411
46. Sefton, AJ, Dreher, B, Harvey, A Visual system. In: Paxinos, G eds. (2004) The Rat Nervous System. Elsevier Academic Press, San Diego, pp. 1083-1165
47. Rapaport, DH, Wong, LL, Wood, ED, Yasumura, D, LaVail, MM (2004) Timing and topography of cell genesis in the rat retina. J Comp Neurol 474: pp. 304-324
48. Agathocleous, M, Harris, WA Cell determination. In: Sernagor, E, Eglen, S, Harris, B, Wong, R eds. (2006) Retinal Development. Cambridge University Press, Cambridge, pp. 75-98
49. Rapaport, DH Retinal Neurogenesis. In: Sernagor, E, Eglen, S, Harris, B, Wong, R eds. (2006) Retinal Development. Cambridge University Press, Cambridge, pp. 30-58
50. Chan-Ling, T, Chu, Y, Baxter, L, Weible Ii, M, Hughes, S (2009) In vivo characterization of astrocyte precursor cells (APCs) and astrocytes in developing rat retinae: differentiation, proliferation, and apoptosis. Glia 57: pp. 39-53
51. Dallimore, EJ, Park, KK, Pollett, MA, Taylor, JSH, Harvey, AR (2010) The life, death and regenerative ability of immature and mature rat retinal ganglion cells are influenced by their birthdate. Exp Neurol 225: pp. 353-365
52. Dreher, B, Potts, RA, Bennett, MR (1983) Evidence that the early postnatal reduction in the number of rat retinal ganglion cells is due to a wave of ganglion cell death. Neurosci Lett 36: pp. 255-260
53. Horsburgh, GM, Sefton, AJ (1987) Cellular degeneration and synaptogenesis in the developing retina of the rat. J Comp Neurol 263: pp. 553-566
54. Potts, RA, Dreher, B, Bennett, MR (1982) The loss of ganglion cells in the developing retina of the rat. Dev Brain Res 3: pp. 481-486
55. Crespo, D, O鈥橪eary, DD, Cowan, WM (1985) Changes in the numbers of optic nerve fibers during late prenatal and postnatal development in the albino rat. Brain Res 351: pp. 129-134
56. Perry, VH, Henderson, Z, Linden, R (1983) Postnatal changes in retinal ganglion cell and optic axon populations in the pigmented rat. J Comp Neurol 219: pp. 356-368
57. Fruttiger, M (2007) Development of the retinal vasculature. Angiogenesis 10: pp. 77-88
58. Dhingra, NK, Ramamohan, Y, Raju, TR (1997) Developmental expression of synaptophysin, synapsin I and syntaxin in the rat retina. Brain Res Dev Brain Res 102: pp. 267-273
59. Sarthy, PV, Bacon, W (1985) Developmental expression of a synaptic vesicle-specific protein in the rat retina. Dev Biol 112: pp. 284-291
60. Kapfhammer, JP, Christ, F, Schwab, ME (1994) The expression of GAP-43 and synaptophysin in the developing rat retina. Brain Res Dev Brain Res 80: pp. 251-260
61. Weidman, TA, Kuwabara, T (1968) Postnatal development of the rat retina. An electron microscopic study. Arch Ophthalmol 79: pp. 470-484
62. Olney, JW (1968) An electron microscopic study of synapse formation, receptor outer segment development, and other aspects of developing mouse retina. Invest Ophthalmol 7: pp. 250-268
63. Altman, J, Bayer, SA (1981) Time of origin of neurons of the rat superior colliculus in relation to other components of the visual and visuomotor pathways. Exp Brain Res 42: pp. 424-434
64. Mustari, MJ, Lund, RD, Graubard, K (1979) Histogenesis of the superior colliculus of the albino rat: a tritiated thymidine study. Brain Res 164: pp. 39-52
65. Giordano, DL, Murray, M, Cunningham, TJ (1980) Naturally occurring neuron death in the optic layers of superior colliculus of the postnatal rat. J Neurocytol 9: pp. 603-614
66. Giordano, DL, Cunningham, TJ (1982) Optic afferents, neuron maturation, and neuron survival in the rat superior colliculus. Brain Res 256: pp. 365-368
67. Warton, SS, Jones, DG (1985) Postnatal development of the superficial layers in the rat superior colliculus: a study with Golgi-Cox and Kluver-Barrera techniques. Exp Brain Res 58: pp. 490-502
68. Warton, SS, McCart, R (1989) Synaptogenesis in the stratum griseum superficiale of the rat superior colliculus. Synapse 3: pp. 136-148
69. Lund, RD, Lund, JS (1972) Development of synaptic patterns in the superior colliculus of the rat. Brain Res 42: pp. 1-20
70. Bunt, SM, Lund, RD, Land, PW (1983) Prenatal development of the optic projection in albino and hooded rats. Brain Res 282: pp. 149-168
71. Dallimore, EJ, Cui, Q, Beazley, LD, Harvey, AR (2002) Postnatal innervation of the rat superior colliculus by axons of late-born retinal ganglion cells. Eur J Neurosci 16: pp. 1295-1304
72. Thong, IG, Dreher, B (1987) The development of the corticotectal pathway in the albino rat: transient projections from the visual and motor cortices. Neurosci Lett 80: pp. 275-282
73. Winter, F, Oudega, M, Lankhorst, AJ, Hamers, FP, Blits, B, Ruitenberg, MJ, Pasterkamp, RJ, Gispen, WH, Verhaagen, J (2002) Injury-induced class 3 semaphorin expression in the rat spinal cord. Exp Neurol 175: pp. 61-75
74. Wit, J, Winter, F, Klooster, J, Verhaagen, J (2005) Semaphorin 3A displays a punctate distribution on the surface of neuronal cells and interacts with proteoglycans in the extracellular matrix. Mol Cell Neurosci 29: pp. 40-55
75. Davenport, RW, Thies, E, Cohen, ML (1999) Neuronal growth cone collapse triggers lateral extensions along trailing axons. Nat Neurosci 2: pp. 254-259
76. Tran, TS, Kolodkin, AL, Bharadwaj, R (2007) Semaphorin regulation of cellular morphology. Annu Rev Cell Dev Biol 23: pp. 263-292
77. Torre, ER, Gutekunst, C-A, Gross, RE (2010) Expression by midbrain dopamine neurons of Sema3A and 3F receptors is associated with chemorepulsion in vitro but a mild in vivo phenotype. Mol Cell Neurosci 44: pp. 135-153
78. Buehler, A, Sitaras, N, Favret, S, Bucher, F, Berger, S, Pielen, A, Joyal, JS, Juan, AM, Martin, G, Schlunck, G, Agostini, HT, Klagsbrun, M, Smith, LE, Sapieha, P, Stahl, A (2013) Semaphorin 3F forms an anti-angiogenic barrier in outer retina. FEBS Lett 587: pp. 1650-1655
79. Edwards, MA, Caviness, VS, Schneider, GE (1986) Development of cell and fiber lamination in the mouse superior colliculus. J Comp Neurol 248: pp. 395-409
80. Shirvan, A, Kimron, M, Holdengreber, V, Ziv, I, Ben-Shaul, Y, Melamed, S, Melamed, E, Barzilai, A, Solomon, AS (2002) Anti-semaphorin 3A antibodies rescue retinal ganglion cells from cell death following optic nerve axotomy. J Biol Chem 277: pp. 49799-49807
81. Shirvan, A, Ziv, I, Fleminger, G, Shina, R, He, Z, Brudo, I, Melamed, E, Barzilai, A (1999) Semaphorins as mediators of neuronal apoptosis. J Neurochem 73: pp. 961-971
82. Gagliardini, V, Fankhauser, C (1999) Semaphorin III can induce death in sensory neurons. Mol Cell Neurosci 14: pp. 301-316
83. Ben-Zvi, A, Manor, O, Schachner, M, Yaron, A, Tessier-Lavigne, M, Behar, O (2008) The Semaphorin receptor PlexinA3 mediates neuronal apoptosis during dorsal root ganglia development. J Neurosci 28: pp. 12427-12432
84. Bagnard, D, Vaillant, C, Khuth, ST, Dufay, N, Lohrum, M, P眉schel, AW, Belin, MF, Bolz, J, Thomasset, N (2001) Semaphorin 3A-vascular endothelial growth factor-165 balance mediates migration and apoptosis of neural progenitor cells by the recruitment of shared receptor. J Neurosci 21: pp. 3332-3341
85. Bagnard, D, Sainturet, N, Meyronet, D, Perraut, M, Miehe, M, Roussel, G, Aunis, D, Belin, MF, Thomasset, N (2004) Differential MAP kinases activation during semaphorin3A-induced repulsion or apoptosis of neural progenitor cells. Mol Cell Neurosci 25: pp. 722-731
86. Schlomann, U, Schwamborn, JC, Muller, M, Fassler, R, P眉schel, AW (2009) The stimulation of dendrite growth by Sema3A requires integrin engagement and focal adhesion kinase. J Cell Sci 122: pp. 2034-2042
87. Polleux, F, Morrow, T, Ghosh, A (2000) Semaphorin 3A is a chemoattractant for cortical apical dendrites. Nature 404: pp. 567-573
88. Morita, A, Yamashita, N, Sasaki, Y, Uchida, Y, Nakajima, O, Nakamura, F, Yagi, T, Taniguchi, M, Usui, H, Katoh-Semba, R, Takei, K, Goshima, Y (2006) Regulation of dendritic branching and spine maturation by semaphorin3A-Fyn signaling. J Neurosci 26: pp. 2971-2980
89. Vo, T, Carulli, D, Ehlert, EME, Kwok, JCF, Dick, G, Mecollari, V, Moloney, EB, Neufeld, G, Winter, F, Fawcett, JW, Verhaagen, J (2013) The chemorepulsive axon guidance protein semaphorin3A is a constituent of perineuronal nets in the adult rodent brain. Mol Cell Neurosci 56: pp. 186-200
90. Cioni, JM, Telley, L, Saywell, V, Cadilhac, C, Jourdan, C, Huber, AB, Huang, JZ, Jahannault-Talignani, C, Ango, F (2013) SEMA3A signaling controls layer-specific interneuron branching in the cerebellum. Curr Biol 23: pp. 850-861
91. Uesaka, N, Uchigashima, M, Mikuni, T, Nakazawa, T, Nakao, H, Hirai, H, Aiba, A, Watanabe, M, Kano, M (2014) Retrograde semaphorin signaling regulates synapse elimination in the developing mouse brain. Science 344: pp. 1020-1023
92. Demyanenko, GP, Maness, PF (2003) The L1 cell adhesion molecule is essential for topographic mapping of retinal axons. J Neurosci 23: pp. 530-538
93. Buhusi, M, Schlatter, MC, Demyanenko, GP, Thresher, R, Maness, PF (2008) L1 interaction with ankyrin regulates mediolateral topography in the retinocollicular projection. J Neurosci 28: pp. 177-188
94. Gong, S, Zheng, C, Doughty, ML, Losos, K, Didkovsky, N, Schambra, UB, Nowak, NJ, Joyner, A, Leblanc, G, Hatten, ME, Heintz, N (2003) A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature 425: pp. 917-925
95. Low, LK, Liu, XB, Faulkner, RL, Coble, J, Cheng, HJ (2008) Plexin signaling selectively regulates the stereotyped pruning of corticospinal axons from visual cortex. Proc Natl Acad Sci U S A 105: pp. 8136-8141
96. Sharma, A, Pollett, MA, Plant, GW, Harvey, AR (2012) Changes in mRNA expression of class 3 semaphorins and their receptors in the adult rat retino-collicular system after unilateral optic nerve injury. Invest Ophthalmol Vis Sci 53: pp. 8367-8377
97. Ramakers, C, Ruijter, JM, Deprez, RH, Moorman, AF (2003) Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci Lett 339: pp. 62-66
98. Ruijter, JM, Ramakers, C, Hoogaars, WM, Karlen, Y, Bakker, O, Hoff, MJ, Moorman, AF (2009) Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data. Nucleic Acids Res 37: pp. e45
99. Schefe, JH, Lehmann, KE, Buschmann, IR, Unger, T, Funke-Kaiser, H (2006) Quantitative real-time RT-PCR data analysis: current concepts and the novel 鈥済ene expression鈥檚 CT difference鈥?formula. J Mol Med (Berl) 84: pp. 901-910
100. Vandesompele, J, Preter, K, Pattyn, F, Poppe, B, Roy, N, Paepe, A, Speleman, F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: pp. Research0034
101. Chen, H, Chedotal, A, He, Z, Goodman, CS, Tessier-Lavigne, M (1997) Neuropilin-2, a novel member of the neuropilin family, is a high affinity receptor for the semaphorins Sema E and Sema IV but not Sema III. Neuron 19: pp. 547-559
102. Meyer-Franke, A, Kaplan, MR, Pfieger, FW, Barres, BA (1995) Characterization of the signaling interactions that promote the survival and growth of developing retinal ganglion cells in culture. Neuron 15: pp. 805-819
103. Kapfhammer, JP, Xu, H, Raper, JA (2007) The detection and quantification of growth cone collapsing activities. Nat Protoc 2: pp. 2005-2011 - 刊物主题：Developmental Biology; Animal Models; Life Sciences, general;
- 出版者：BioMed Central
- ISSN：1471-213X
文摘
Background Members of the Semaphorin 3 family (Sema3s) influence the development of the central nervous system, and some are implicated in regulating aspects of visual system development. However, we lack information about the timing of expression of the Sema3s with respect to different developmental epochs in the mammalian visual system. In this time-course study in the rat, we document for the first time changes in the expression of RNAs for the majority of Class 3 Semaphorins (Sema3s) and their receptor components during the development of the rat retina and superior colliculus (SC). Results During retinal development, transcript levels changed for all of the Sema3s examined, as well as Nrp2, Plxna2, Plxna3, and Plxna4a. In the SC there were also changes in transcript levels for all Sema3s tested, as well as Nrp1, Nrp2, Plxna1, Plxna2, Plxna3, and Plxna4a. These changes correlate with well-established epochs, and our data suggest that the Sema3s could influence retinal ganglion cell (RGC) apoptosis, patterning and connectivity in the maturing retina and SC, and perhaps guidance of RGC and cortical axons in the SC. Functionally we found that SEMA3A, SEMA3C, SEMA3E, and SEMA3F proteins collapsed purified postnatal day 1 RGC growth cones in vitro. Significantly this is a developmental stage when RGCs are growing into and within the SC and are exposed to Sema3 ligands. Conclusion These new data describing the overall temporal regulation of Sema3 expression in the rat retina and SC provide a platform for further work characterising the functional impact of these proteins on the development and maturation of mammalian visual pathways.