Comparison of ginsenoside composition in native roots and cultured callus cells of Panax quinquefolium L.
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- 作者：Juan Wang (1) (2)
Hui Liu (1)
Wen-Yuan Gao (1) (2)
Liming Zhang (1) - 关键词：Panax quinquefolium L. ; Suspension cell ; Ginsenoside ; HPLC–ESI ; MSn ; Antioxidant
- 刊名：Acta Physiologiae Plantarum
- 出版年：2013
- 出版时间：April 2013
- 年：2013
- 卷：35
- 期：4
- 页码：1363-1366
- 全文大小：219KB
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Hui Liu (1)
Wen-Yuan Gao (1) (2)
Liming Zhang (1)
1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China
2. Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China
文摘
In order to compare the ginsenoside composition in native Panax quinquefolium and in suspension cultured cells derived from root callus, HPLC–ESI-MSn analysis was performed. Under the present HPLC–ESI-MSn conditions, ten ginsenosides from native root were acquired in the positive and negative ion modes, namely Rg1, Re, Ro, malonyl-Rb1, Rf, Rb1, Rc, Rb2, Rb3 and Rd. Only four ginsenosides (Rg1, Re, Rf and Rb1) were identified from callus cells. Radical scavenging activity of P. quinquefolium callus cells with 250?mg?l? methanolic extract on 1,1-diphenyl-2-picrylhydrazyl (DPPH) was 55.72?%, while only 6.31?% DPPH inhibition was obtained in native root.