Identification and validation of sex-linked SCAR markers in dioecious Hippophae rhamnoides L. (Elaeagnaceae)

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• 作者：Girish Korekar (1)
  Ram Kumar Sharma (2)
  Rahul Kumar (2)
  Meenu (3)
  Naveen C. Bisht (3)
  Ravi B. Srivastava (1)
  Paramvir Singh Ahuja (2)
  Tsering Stobdan (1) ts_mbb@yahoo.com
• 关键词：Dioecious – ; Hippophae – ; RAPD – ; SCAR – ; Sex determination
• 刊名：Biotechnology Letters
• 出版年：2012
• 出版时间：May 2012
• 年：2012
• 卷：34
• 期：5
• 页码：973-978
• 全文大小：244.3 KB
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• 作者单位：1. Defence Institute of High Altitude Research, Defence R & D Organisation, Leh-Ladakh, 194101 India2. Institute of Himalayan Bioresource Technology, Palampur, 176061 India3. National Institute of Plant Genome Research, New Delhi, 110067 India
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

The actinorhizal plant seabuckthorn (Hippophae rhamnoides L., Elaeagnaceae) is a wind pollinated dioecious crop. To distinguish male genotypes from female genotypes early in the vegetative growth phase, we have developed robust PCR-based marker(s). DNA bulk samples from 20 male and 20 female plants each were screened with 60 RAPD primers. Two primers, OPA-04 and OPT-06 consistently amplified female-specific (FS) polymorphic fragments of 1,164 and 868 bp, respectively, that were absent in the male samples. DNA sequence of the two markers did not exhibit significant similarity to previously characterized sequences. A sequence-characterized amplified region marker HrX1 (JQ284019) and HrX2 (JQ284020) designed for the two fragments, continued to amplify the FS allele in 120 female plants but not in 100 male plants tested in the current study. Thus, HrX1 and HrX2 are FS markers that can determine the sex of seabuckthorn plants in an early stage and expedite cultivations for industrial applications.