Deep sequencing and analysis of small RNAs in sweet orange grafted on sour orange infected with two citrus tristeza virus isolates prevalent in Sicily

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• 作者：Grazia Licciardello ; Giuseppe Scuderi ; Rosario Ferraro…
• 刊名：Archives of Virology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：160
• 期：10
• 页码：2583-2589
• 全文大小：1,252 KB
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  13.Licciardello G, Russo M, Daden M, Bar-Joseph M, Catara A (2015) Capillary electrophoresis-single-strand conformation polymorphisms and multiple molecular marker (MMM) genotyping allow a rapid differentiation of Citrus tristeza virus (CTV) isolates. Acta Hortic 1065:773-80
  14.Garnsey SM, Civerolo EL, Gumpf DJ, Paul C, Hilf ME, Lee RF, Brlansky RH, Yokomi RK, Hartung JS (2005) Biological characterisation of an international collection of Citrus tristeza virus (CTV) isolates. In: Proceedings of the 16th conference of the international organisation of citrus virologists. IOCV, Riverside, pp 75-3
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• 作者单位：Grazia Licciardello (1)
  Giuseppe Scuderi (1)
  Rosario Ferraro (1)
  Annalisa Giampetruzzi (2)
  Marcella Russo (1)
  Alessandro Lombardo (1)
  Domenico Raspagliesi (1)
  Moshe Bar-Joseph (3)
  Antonino Catara (1)
  
  1. Parco Scientifico e Tecnologico della Sicilia, z.i. Blocco Palma I, Str.?le Lancia 57, 95121, Catania, Italy
  2. CNR, Institute for Sustainable Plant Protection, Via Amendola 165/A, 70126, Bari, Italy
  3. Gimlaotec, 8 Hazanchanim St., Rehovot, 76211, Israel
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798

文摘

Two representative isolates of a citrus tristeza virus population in Sicily, SG29 (aggressive) and Bau282 (mild), were sequenced via viral small RNAs (vsRNA) produced in budlings of sweet orange grafted on sour orange. Phylogenetic relationships with Mediterranean and exotic isolates revealed that SG29 clustered within the “VT-Asian-subtype, whereas Bau282 belonged to the cluster T30. The study confirms that molecular data need to be integrated with bio-indexing in order to obtain adequate information for risk assessment.