Characterization of Burkholderia glumae BGR1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR), the terminal enzyme in 2-C-methyl-d
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- 作者：Moonhyuk Kwon (1) <br> Bok-Kyu Shin (2) <br> Jaekyoung Lee (3) <br> Jaehong Han (2) <br> Soo-Un Kim (1) <br>
- 关键词：Burkholderia glumae ; complementation assay ; dimenthylally diphosphate (DMAPP) ; 4 ; hydroxy ; 3 ; methylbut ; 2 ; enyl diphosphate reductase (HDR) ; isopentenyl diphosphate (IPP) ; 2 ; C ; methyl ; d ; erythritol 4 ; phosphate (MEP) pathway
- 刊名：Journal of the Korean Society for Applied Biological Chemistry
- 出版年：2013
- 出版时间：February 2013
- 年：2013
- 卷：56
- 期：1
- 页码：35-40
- 全文大小：391KB
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- 作者单位：Moonhyuk Kwon (1) <br> Bok-Kyu Shin (2) <br> Jaekyoung Lee (3) <br> Jaehong Han (2) <br> Soo-Un Kim (1) <br><br>1. Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-951, Republic of Korea <br> 2. Metalloenzyme Research Group, Department of Biotechnology, Chung-Ang University, Anseong, 456-756, Republic of Korea <br> 3. R&D Center, AmorePacific Corporation, Yongin, 446-729, Republic of Korea <br>
- ISSN：2234-344X
文摘
4-Hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR) is the ultimate enzyme in 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway converting (E)-4-hydroxy-3-methylbut-2-enyl pyrophosphate (HMBPP) into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Burkholderia glumae, a Gram-negative rice-pathogenic bacterium, harbors 2 hdr genes and lacks isopentenyl diphosphate isomerase (idi). Both HDR enzymes could complement E. coli hdr deletion mutant (DYTL1). Both of the recombinant HDR proteins, BgHDR1 and BgHDR2, catalyzed reduction of HMBPP into IPP and DMAPP at a ratio of 2:1, in contrast to 5:1 ratio of other bacterial HDRs so far characterized. The k catb> and K mb> values of BgHDR1 and BgHDR2 were 187.0 min? and 6.0 μM and 66.6 min? and 21.2 μM, respectively. Physiological significance of the kinetic properties was discussed.