Impaired glycocalyx barrier properties contribute to enhanced intimal low-density lipoprotein accumulation at the carotid artery bifurcation in mice
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- 作者：Bernard M. van den Berg (1)
Jos A. E. Spaan (3)
Hans Vink (1) (2) - 关键词：Carotid artery bifurcation ; Glycocalyx ; Heparan sulfate ; Hyaluronan ; Permeability
- 刊名：Pfl眉gers Archiv - European Journal of Physiology
- 出版年：2009
- 出版时间：April 2009
- 年：2009
- 卷：457
- 期：6
- 页码：1199-1206
- 全文大小：307KB
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Jos A. E. Spaan (3)
Hans Vink (1) (2)
1. Department of Physiology, CARIM, Maastricht University, Universiteitssingel 50, 6229 ER, P.O. Box 616, 6200 MD, Maastricht, The Netherlands
3. Department of Medical Physics, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
2. Department of Vascular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands - ISSN：1432-2013
文摘
The glycocalyx contributes to the barrier properties of vascular endothelium, and recently, we reported using electron microscopy that glycocalyx is diminished at lesion prone sites in arterial bifurcations in mice. In the present study, we examined using confocal microscopy the dimension and composition of the endothelial glycocalyx at low- and high-risk atherogenic regions within the common carotid (common) and internal carotid branch (sinus) of C57BL/6J mice and compared dimensional variations with its ability to limit transendothelial leakage of low-density lipoprotein (LDL). Confocal laser scanning microscopy of arterial surfaces stained for heparan sulfate and hyaluronan revealed thinner glycocalyces at the sinus region (2.2?±-.7 and 2.3?±-.7?μm, respectively; P-lt;-.05) than the glycocalyx thickness at the common region (4.3?±-.6 and 4.3?±-.6?μm, respectively). This thinner glycocalyx was associated with impaired LDL retention by the glycocalyx resulting in a two to three times increase in intimal accumulation of LDL 15?min after i.v. bolus administration: 10.8?±-.6 vs. 4.0?±-.9?×-0,000?a.u. (sinus vs. common, P-lt;-.05). These results indicate that impaired glycocalyx barrier properties may contribute to transendothelial leakage of atherogenic LDL at lesion prone arterial sites.