A light and electron microscopic study of Pelomyxa secunda (Gruber, 1884) comb. nov. (Archamoebae, Pelobiontida)
文摘
The morphology of the Pelomyxa secunda (Gruber, 1884) comb. nov. pelobiont was studied at light and ultrastructural levels. The locomotive forms are oblong and cigar-shaped. The size range of moving specimens constitutes from 200 to 300 μm. Larger specimens reaching 400 μm are not capable of directed movement. At the sides of the body and at the frontal end, small hyaline pseudopodia could be formed most often that were finger-shaped. The cellular coat is represented by amorphous glycocalyx with a thickness of up to 300 nm. A thin layer of the peripheral cytoplasm without any organelles, vacuoles, endocytobionts, and other inclusions, which is separated from the rest of the cytoplasm by a layer of microfilaments, is below the plasmalemma. P. secunda has two species of obligate prokaryotic endocytobionts located in symbiontophoric vacuoles. Granular nuclei and nucleosomal material are represented by discrete structures of two types differing in size and electron density. The external membrane of the nuclear envelope has from two to three layers of short microtubules parallel to each other and to the nucleus surface. Undulipodia, kinetosomes, and their root derivatives have not been observed.