Cross-reactivity of Antibodies Directed to the Gram-Negative Bacterium Neisseria gonorrhoeae With Heat Shock Protein 60 and ATP-Binding Protein Correlates to Reduced Mitochondrial Activity in HIBCPP Choroid Plexus Papilloma C

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• 作者：B. Reuss ; H. Schroten ; H. Ishikawa ; A. R. Asif
• 关键词：HIBCPP ; Choroid plexus ; Neisseria gonorrhoeae ; Mitochondria ; Hsp60 ; ATPB
• 刊名：Journal of Molecular Neuroscience
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：57
• 期：1
• 页码：123-138
• 全文大小：2,346 KB
• 参考文献：Anglin RE, Garside SL, Tarnopolsky MA, Mazurek MF, Rosebush PI (2012) The psychiatric manifestations of mitochondrial disorders: a case and review of the literature. J Clin Psychiatry 73:506-12CrossRef PubMed
  Anthony SG, Schipper HM, Tavares R, Hovanesian V, Cortez SC, Stopa EG, Johanson CE (2003) Stress protein expression in the Alzheimer-diseased choroid plexus. J Alzheimers Dis 5:171-77PubMed
  Asif AR, Oellerich M, Amstrong VW, Gross U, Reichard U (2010) Analysis of the cellular Aspergillus fumigatus proteome that reacts with sera from rabbits developing an acquired immunity after experimental aspergillosis. Electrophoresis 31:1947-958CrossRef PubMed
  Babulas V, Factor-Litvak P, Goetz R, Schaefer CA, Brown AS (2006) Prenatal exposure to maternal genital and reproductive infections and adult schizophrenia. Am J Psychiatry 163:927-29CrossRef PubMed
  Bae TJ, Kim MS, Kim JW, Kim BW, Choo HJ, Lee JW, Kim KB, Lee CS, Kim JH, Chang SY, Kang CY, Lee SW, Ko YG (2004) Lipid raft proteome reveals ATP synthase complex in the cell surface. Proteomics 4:3536-548CrossRef PubMed
  Berg K, Hansen MB, Nielsen SE (1990) A new sensitive bioassay for precise quantification of interferon activity as measured via the mitochondrial dehydrogenase function in cells (MTT-method). APMIS 1990(982):156-62CrossRef
  Bini L, Pacini S, Liberatori S, Valensin S, Pellegrini M, Raggiaschi R, Pallini V, Baldari CT (2003) Extensive temporally regulated reorganization of the lipid raft proteome following T-cell antigen receptor triggering. Biochem J 369:301-09PubMed Central CrossRef PubMed
  Bollag DM, Edelstein SJ (1994) Isoelectric focusing and two dimensional gel electrophoresis. In: Protein Methods, Chapter 7.Wiley-Liss, Inc.
  Buckman JF, Reynolds IJ (2001) Spontaneous changes in mitochondrial membrane potential in cultured neurons. J Neurosci 21:5054-065PubMed
  Bueno D, Garcia PM, Fernandez J (2014) The embryonic blood cerebrospinal fluid barrier function before the formation of the fetal choroid plexus: role in cerebrospinal fluid formation and homeostasis. Croat Med J 55:306-16PubMed Central CrossRef PubMed
  Campbell GR, Kraytsberg Y, Krishnan KJ, Ohno N, Ziabreva I, Reeve A, Trapp BD, Newcombe J, Reynolds R, Lassmann H, Khrapko K, Turnbull DM, Mahad DJ (2012) Clonally expanded mitochondrial DNA deletions within the choroid plexus in multiple sclerosis. Acta Neuropathol 124:209-20PubMed Central CrossRef PubMed
  Casademont J, Garrabou G, Miró O, López S, Pons A, Bernardo M, Cardellach F (2007) Neuroleptic treatment effect on mitochondrial electron transport chain: peripheral blood mononuclear cells analysis in psychotic patients. J Clin Psychopharmacol 27:284-88CrossRef PubMed
  Clay HB, Sillivan S, Konradi C (2011) Mitochondrial dysfunction and pathology in bipolar disorder and schizophrenia. Int J Dev Neurosci 29:311-24PubMed Central CrossRef PubMed
  Contreras-Shannon V, Heart DL, Paredes RM, Navaira E, Catano G, Maffi SK, Walss-Bass C (2013) Clozapine-induced mitochondria alterations and inflammation in brain and insulin-responsive cells. PLoS One 8, e59012PubMed Central CrossRef PubMed
  Cooperstein SJ, Lazarow A (1953) Studies on the mechanism of Janus green B staining of mitochondria. Exp Cell Res 5:82-7CrossRef PubMed
  Cornford EM, Varesi JB, Hyman S, Damian RT, Raleigh MJ (1997) Mitochondrial content of choroid plexus epithelium. Exp Brain Res 116:399-05CrossRef PubMed
  Cossu D, Cocco E, Paccagnini D, Masala S, Ahmed N, Frau J, Marrosu MG, Sechi LA (2011) Association of Mycobacterium avium subsp. paratuberculosis with multiple sclerosis in Sardinian patients. PLoS One 6, e18482PubMed Central CrossRef PubMed
  Coulombe PA, Wong P (2004) Cytoplasmic intermediate filaments revealed as dynamic and multipurpose scaffolds. Nat Cell Biol 6:699-06CrossRef PubMed
  Dahm L, Klugmann F, Gonzalez-Algaba A, Reuss B (2010) Tamoxifen and raloxifene modulate gap junction coupling during early phases of retinoic acid-dependent neuronal differentiation of NTera2/D1 cells. Cell Biol Toxicol 26:579-91PubMed Central CrossRef PubMed
  Dale RC, Brilot F (2012) Autoimmune basal ganglia disorders. J Child Neurol 27:1470-481CrossRef PubMed
  Damkier HH, Brown PD, Praetorius J (2013) Cerebrospinal fluid secretion by the choroid plexus. Physiol Rev 93:1847-892CrossRef PubMed
  Das B, Mondragon MO, Sadeghian M, Hatcher VB, Norin AJ (1994) A novel ligand in lymphocyte-mediated cytotoxicity: expression of the beta subunit of H+ transporting ATP synthase on the surface of tumor cell lines. J Exp Med 180:273-81CrossRef PubMed
  Davies KM, Strauss M, Daum B, Kief JH, Osiewacz HD, Rycovska A, Zickermann V, Kühlbrandt W (2011) Macromolecular organization of ATP synthase and complex I in whole mitochondria. Proc Natl Acad Sci U S A 108:14121-4126PubMed Central CrossRef PubMed
  Deitiker P, Ashizawa T, Atassi MZ (2000) Antigen mimicry in autoimmune disease. Can immune responses to microbial
• 作者单位：B. Reuss (1)
  H. Schroten (2)
  H. Ishikawa (3)
  A. R. Asif (4)
  
  1. Institute for Neuroanatomy, University Medicine G?ttingen (UMG), Kreuzbergring 36, 37075, G?ttingen, Federal Republic of Germany
  2. University Children’s Hospital Mannheim, Pediatric Infectious Diseases, Heidelberg University, Heidelberg, Germany
  3. The Nippon Dental University, Tokyo, Japan
  4. Institute for Clinical Chemistry, University Medicine G?ttingen (UMG), G?ttingen, Germany
  
• 刊物主题：Neurosciences; Neurochemistry; Cell Biology; Proteomics; Neurology;
• 出版者：Springer US
• ISSN：1559-1166

文摘

Antibacterial antibodies can cause neurologic side-effects by cross-reactivity with cellular antigens. Here we investigated interactions of antibodies to Neisseria gonorrhoeae (α-NG) - maternal infections by which increases the offspring’s risk for later psychosis—with HIBCPP cells, a cell culture model of choroid plexus epithelium. Immunocytochemistry and Western blotting with α-NG, revealed organelle-like intracellular staining in HIBCPP cells, and labelling of several immunoreactive bands in cellular protein. Two-dimensional Western blotting revealed several immunopositive spots, most prominent of which were identified by mass spectrometry as mitochondrially localized proteins heat shock protein 60 (Hsp60) and ATP-binding protein β-subunit (ATPB). Similarly α-NG interacted with commercial samples of these proteins as revealed by Western blotting. Three alternative methods (JC-1, Janus green and MTT staining) revealed α-NG to cause in HIBCPP cells a significant decrease in mitochondrial activity, which could be reverted by neuroleptic drugs. Immunoreactivity of α-NG with choroid plexus epithelium in human post mortem samples suggests in vivo relevance of these findings. Finally, distinctly different staining patterns of antibodies against Neisseria meningitidis (α-NM), confirmed antibody specificity. To our knowledge this is the first report that α-NG cross-reactivity with Hsp60 and ATPB impairs mitochondrial activity in choroid plexus epithelial cells, pathogenetic relevance of which needs further clarification. Keywords HIBCPP Choroid plexus Neisseria gonorrhoeae Mitochondria Hsp60 ATPB