Monitoring surface-assisted biomolecular assembly by means of evanescent-field-coupled waveguide-mode nanobiosensors

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• 作者：Subash C. B. Gopinath ; Koichi Awazu ; Makoto Fujimaki ; Katsuaki Sugimoto ; Yoshimichi Ohki ; Tetsuro Komatsubara ; Junji Tominaga and Penmetcha K. R. Kumar
• 关键词：Biomolecular assembly ; Nanobiosensor ; Evanescent ; field ; coupled waveguide ; SiO2 ; Aptamer
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2009
• 出版时间：May, 2009
• 年：2009
• 卷：394
• 期：2
• 页码：481-488
• 全文大小：447.9 KB

文摘

Biological self-assembly is a natural process that involves various biomolecules, and finding the missing partner in these interactions is crucial for a specific biological function. Previously, we showed that evanescent-field-coupled waveguide-mode sensor in conjunction with a SiO₂ waveguide, the surfaces which contain cylindrical nanometric holes produced by atomic bombardment, allowed us to detect efficiently the biomolecular interactions. In the present studies, we showed that the assembly of biomolecules can be monitored using the evanescent-field-coupled waveguide-mode biosensor and thus provide a methodology in monitoring assembly process in macromolecular machines while they are assembling.