Evaluation of nucleic acid duplex formation on gold over layers in biosensor fabricated using Czochralski-grown single-crystal silicon substrate

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• 作者：Subash C. B. Gopinath ; Ramanujam Kumaresan ; Koichi Awazu ; Makoto Fujimaki ; Minoru Mizuhata ; Junji Tominaga and Penmetcha K. R. Kumar
• 关键词：Spectrophotometry ; Crystal ; Amorphous ; Au layer ; DNA hybridization
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：398
• 期：2
• 页码：751-758
• 全文大小：455.5 KB

文摘

With a view to developing an economical and elegant biosensor chip, we compared the efficiencies of biosensors that use gold-coated single-crystal silicon and amorphous glass substrates. The reflectivity of light over a wide range of wavelengths was higher from gold layer coated single-crystal silicon substrates than from glass substrates. Furthermore, the efficiency of reflection from gold layers of two different thicknesses was examined. The thicker gold layer (100 nm) on the single-crystal silicon showed a higher reflectivity than the thinner gold film (10 nm). The formation of a nucleic acid duplex and aptamer–ligand interactions were evaluated on these gold layers, and a crystalline silicon substrate coated with the 100-nm-thick gold layer is proposed as an alternative substrate for studies of interactions of biomolecules.