Microarray analysis of oral mucosal epithelial cell sheet

详细信息    查看全文

• 作者：Fawzia Bardag-Gorce (1)
  Joan Oliva (1)
  Andrew Wood (1)
  Hope Niihara (1)
  Andrew Makalinao (1)
  Sean Sabino (1)
  Derek Pan (1)
  Jacquelyn Thropay (1)
  Hiroyuki Sota (1)
  Yutaka Niihara (1)
  
• 关键词：oral ; mucosal ; epithelium ; cell sheet ; engineering ; cornea
• 刊名：Tissue Engineering and Regenerative Medicine
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：10
• 期：6
• 页码：362-370
• 全文大小：
• 作者单位：Fawzia Bardag-Gorce (1)
  Joan Oliva (1)
  Andrew Wood (1)
  Hope Niihara (1)
  Andrew Makalinao (1)
  Sean Sabino (1)
  Derek Pan (1)
  Jacquelyn Thropay (1)
  Hiroyuki Sota (1)
  Yutaka Niihara (1)
  
  1. LA BioMed Institute at Harbor UCLA Medical Center, Torrance, California, 90502, USA
  
• ISSN：2212-5469

文摘

Corneal epithelium regeneration using autologous oral mucosal epithelial cell sheet is a successful new approach in corneal therapy. In the present study, gene expression profiling was performed to characterize engineered cell sheets. Cell sheets were obtained by culturing isolated rabbit oral mucosal epithelial cells on a thermoresponsive cultureware (UpCell?, CellSeed Inc. Japan). H&E staining of cell sheets showed a multistratified epithelium, similar to corneal epithelium. DeltaN-p63 stained positive in the basal cells, indicating that cell sheets have renewal capacity. Microarray analysis of these cell sheets showed that only 160 genes out of 43,000 rabbit probes listed on the microarray chip were identified. We first identified the extracellular matrix group of genes and found that matrix metalloproteinase MMP-1, MMP-3 and MMP-12, known to promote angiogenesis, were down regulated, while MMP-13 and collagen type VIII alpha 1 (COL8A1), proteins involved in wound healing, were up regulated. Tissue inhibitors of metalloproteinase TIMP-1 and TIMP-3, anti-angiogenic factors, were also identified. Gap junction protein A7 (GJA7 or Connexin 45) was found up regulated, indicating that cell sheets have developed well preserved cell-cell interactions. Alcohol dehydrogenase 5 (ADH class III) and aldehyde dehydrogenase (ALDH1A1), involved in protecting the cornea against oxidative stress induced by UV radiation, were also found up regulated. In conclusion, microarray analysis has led us to identify new target molecules and their subsequent biochemical analysis indicated how the composite cell sheets are advantageous to the original isolated cells in terms of the integrity and potency of corneal epithelial grafts without any scaffolds.