Water metabolism in cells of Saccharomyces cerevisiae of races Y-3137 and Y-3327, according to pulsed-field gradient NMR data

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• 作者：I. A. Avilova ; S. G. Vasil’ev ; L. V. Rimareva…
• 关键词：yeast cells ; self ; diffusion ; pulsed field gradient NMR ; permeability
• 刊名：Russian Journal of Physical Chemistry A, Focus on Chemistry
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：89
• 期：4
• 页码：710-714
• 全文大小：394 KB
• 参考文献：1. Rimareva, L V (2003) Theoretical and Practical Principles of Yeast Biotechnology.
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  7. Kadieva, A T, Overchenko, M B, Ignatova, N I, Rimareva, L V (2003) Proizv. Spirta Likero-Vodoch. Izdel., No. 4.
  8. Rimareva, L V, Overchenko, M B, Ignatova, N I, Serba, E M (2012) Proizv. Spirta Likero-Vodoch. Izdel., No. 3.
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Physical Chemistry
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1531-863X

文摘

The self-diffusion of water in cells of Saccharomyces cerevisiae of races Y-3137 and Y-3327 is studied by means of pulsed-field gradient (PFG) NMR. Three types of water are detected that differ by their self-diffusion coefficients (SDCs): free, intercellular, and intracellular. It is found that the self-diffusion of intercellular and intracellular water is restricted. The size and permeability of the cells of yeasts with different cultivation times (24 and 48 h) is determined by analyzing the dependences of the self-diffusion coefficients of intracellular water on the interval between pulses of the magnetic field gradient.