Development of a Method for the Analysis of Sterols in Sterol-Enriched Deli-Style Turkey with GC-FID

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• 作者：S. Grasso ; N. P. Brunton ; F. J. Monahan ; S. M. Harrison
• 关键词：β ; Sitosterol ; Campesterol ; Stigmasterol ; Turkey meat ; GC ; flame ionisation detection ; Relative response factor
• 刊名：Food Analytical Methods
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：9
• 期：3
• 页码：724-728
• 全文大小：325 KB
• 参考文献：Clement LM, Hansen SL, Costin CD, Perri GL (2010) Quantitation of sterols and sterol esters in fortified foods and beverages by GC/FID. J Am Oil Chem Soc 87:973–980. doi:10.​1007/​s11746-010-1579-9 CrossRef
  Du M, Ahn D (2002) Simultaneous analysis of tocopherols, cholesterol, and phytosterols using gas chromatography. J Food Sci 67:1696–1700CrossRef
  European Food Safety Authority Panel on Dietetic Products, Nutrition and Allergies (NDA) (2010) Scientific Opinion on the substantiation of health claims related to plant sterols and plant stanols and maintenance of normal blood cholesterol concentrations (ID 549, 550, 567, 713, 1234, 1235, 1466, 1634, 1984, 2909, 3140), and maintenance of normal prostate size and normal urination (ID 714, 1467, 1635) pursuant to Article 13(1) of Regulation (EC) No 1924/2006. EFSA Journal: 22 pp. doi:10.​2903/​j.​efsa.​2010.​1813
  Laakso PH (2014) Determination of plant stanols and plant sterols in phytosterol enriched foods with a gas chromatographic-flame ionization detection method: NMKL collaborative study. J AOAC Int 97:1097–1108CrossRef
  Rome K, McIntyre A (2012) Intelligent use of Relative Response Factors in Gas Chromatography-Flame Ionisation Detection Chromatography today:4
  Srigley CT, Haile EA (2015) Quantification of plant sterols/stanols in foods and dietary supplements containing added phytosterols. J Food Compos Anal 40:163–176. doi:10.​1016/​j.​jfca.​2015.​01.​008 CrossRef
  U.S. Food and Drug Administration, Department of Health and Human Services (2001) Bioanalytical Method Validation, Guidance for Industry
  
• 作者单位：S. Grasso (1)
  N. P. Brunton (1)
  F. J. Monahan (1)
  S. M. Harrison (1)
  
  1. UCD School of Agriculture and Food Science, University College Dublin, Dublin 4, Ireland
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X

文摘

Plant sterols have been recognised by the European Food Safety Authority for their cholesterol-lowering properties and are currently added to several food formulations. The objective of this study was to develop a method based on formation of sterol trimethylsilyl ether derivatives and separation and quantification by GC for the determination of three phytosterols (β-sitosterol, stigmasterol and campesterol) in sterol-enriched deli-style turkey. The assay was linear (concentration range 4.3–172.1 μg/ml, R 2 ≥ 0.9868) and accuracy and precision were within the acceptance criteria of the U.S. Food and Drug Administration (USFDA) guidelines for method validation, set at <20 % RSD at the lower limit of quantification (LLOQ) and <15 % RSD for all other standards. Accuracy measured by relative response factor (RRF) also met the USFDA validation criteria. No matrix effects were observed. The response factors (RFs) of the three sterols differed significantly to that of the internal standard (ISTD) used, leading to RRF dissimilar to 1 (campesterol = 1.0167, stigmasterol = 1.4458, β-sitosterol = 0.9029). This method is suitable for quantification in meat matrix, and it has been successfully applied to the determination of sterols in sterol-enriched deli-style turkey (21 mg sterols/0.5 g sample). Keywords β-Sitosterol Campesterol Stigmasterol Turkey meat GC-flame ionisation detection Relative response factor