Structure elucidation of NeuAc, NeuGc and Kdn-containing O-glycans released from Triturus alpestris oviductal mucins

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• 作者：Doina Florea ; Emmanuel Maes ; Yann Guérardel ; Adeline Page ; Jean-Pierre Zanetta ; Dan Cogalniceanu and Gérard Strecker
• 关键词：Amphibia ; Triturus alpestris ; Jelly coat ; Mucin ; O ; glycan ; Poly LacdiNAc ; NMR ; ESI ; MS/MS
• 刊名：Glycoconjugate Journal
• 出版年：2006
• 出版时间：July, 2006
• 年：2006
• 卷：23
• 期：5-6
• 页码：377-399
• 全文大小：1,433 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-4986

文摘

Eggs from Amphibia are surrounded by several layers of jelly that are needed for proper fertilization. Jelly coat is composed of highly glycosylated mucin-type glycoproteins containing up to 60% of carbohydrates, which display a remarkable species-specificity. This material obtained from Triturus alpestris was submitted to reductive β -elimination and the released oligosaccharide-alditols were further fractionated by HPLC. Structural characterization was performed through a combination of two dimensional 1H-1H and 1H-13C NMR and ESI-MS/MS analysis. Numerous carbohydrate chains are characterized by the presence of the Cad (Sda) determinant, including respectively NeuAc, NeuGc or Kdn as a sialic acid. But the most significant O-glycan sequences which mark the difference between the jelly of T. alpestris and other studies amphibian jellies are polymers of GalNAc(β 1-4)GlcNAc (LacdiNAc) which form part of the following sequence: HSO₃(4)(GalNAcβ 1-4GlcNAcβ 1-3)_1-3GalNAcβ 1-4(GlcNAcβ 1-3)_0-1GlcNAcβ 1-6GalNAc-ol