CLIP: viewing the RNA world from an RNA-protein interactome perspective

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• 作者：Yin Zhang (1)
  ShuJuan Xie (1)
  Hui Xu (1)
  LiangHu Qu (1)
  
  1. Key Laboratory of Gene Engineering of Ministry of Education ; State Key Laboratory for Biocontrol ; Sun Yat-Sen University ; Guangzhou ; 510275 ; China
  
• 关键词：CLIP ; CLASH ; RPBs ; ncRNAs ; functional RNomics
• 刊名：Science China Life Sciences
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：58
• 期：1
• 页码：75-88
• 全文大小：1,007 KB
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• 刊物主题：Life Sciences, general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-1889

文摘

The pervasive transcription of the genome creates many types of non-coding RNAs (ncRNAs). However, we know very little regarding the functions and the regulatory mechanisms of these ncRNAs. Exploring the interactions of RNA and RNA binding proteins (RBPs) is vital because it can allow us to truly understand how these ncRNAs behave in vivo. High-throughput sequencing of RNA isolated by cross-linking immunoprecipitation (HITS-CLIP or CLIP-seq) and its variants have been successfully used as systemic techniques to study RBP binding sites. In this review, we will explain the major differences between the CLIP techniques, summarize successful applications of these techniques, discuss limitations of CLIP, present some suggested solutions and project their promising future roles in studying the RNA world.