Transcriptional profiling of genes involved in ascorbic acid biosynthesis, recycling, and degradation during three leaf developmental stages in celery
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- 作者：Wei Huang ; Guang-Long Wang ; Hui Li ; Feng Wang…
- 关键词：Apium graveolens L. ; Ascorbic acid ; Development ; Gene expression ; Leaf ; Metabolism
- 刊名：Molecular Genetics and Genomics
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：291
- 期：6
- 页码：2131-2143
- 全文大小：2,699 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Cell Biology
Biochemistry
Microbial Genetics and Genomics - 出版者：Springer Berlin / Heidelberg
- ISSN：1617-4623
- 卷排序：291
文摘
Ascorbic acid (AsA) is an important nutrient in the human body and performs various healthy functions. With considerable medicinal properties, celery (Apium graveolens L.) could be a good source of AsA for human health. However, the biosynthetic, recycling, and degradation pathways of AsA in celery have yet to be characterized. To study the metabolic pathways involved in AsA, the genes involved in AsA biosynthesis, recycling, and degradation were isolated from celery, and their expression profiles and AsA levels were analyzed in the leaf blades and petioles of two celery varieties at three different growth stages. AsA levels were higher in ‘Ventura’ compared with ‘Liuhehuangxinqin’ in both tissues possibly because of different transcription levels of genes, such as l-galactose dehydrogenase (GalDH), l-galactono-1,4-lactone dehydrogenase (GalLDH), and glutathione reductase (GR). Results revealed that the d-mannose/l-galactose pathway may be the predominant pathway in celery, and the d-galacturonic acid pathway appeared to contribute largely to AsA accumulation in petioles than in leaf blades in ‘Liuhehuangxinqin.’ AsA contents are regulated by complex regulatory mechanisms and vary at different growth stages, tissues, and varieties in celery. The results provide novel insights into AsA metabolic pathways in leaf during celery growth and development.