Characterization and application of bioflocculant prepared by Rhodococcus erythropolis using sludge and livestock wastewater as cheap culture media
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- 作者：Lanyan Peng (1) (3)
Chunping Yang (1) (2) (3)
Guangming Zeng (1) (3)
Lu Wang (1) (3)
Chuanhua Dai (1) (3)
Zhiyong Long (1) (3)
Hongyu Liu (1) (3)
Yuanyuan Zhong (1) (3) - 关键词：Pretreatment ; Bioflocculant ; Polysaccharides ; Excess sludge ; Rhodococcus erythropolis ; Response surface methodology
- 刊名：Applied Microbiology and Biotechnology
- 出版年：2014
- 出版时间：August 2014
- 年：2014
- 卷：98
- 期：15
- 页码：6847-6858
- 全文大小：986 KB
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Chunping Yang (1) (2) (3)
Guangming Zeng (1) (3)
Lu Wang (1) (3)
Chuanhua Dai (1) (3)
Zhiyong Long (1) (3)
Hongyu Liu (1) (3)
Yuanyuan Zhong (1) (3)
1. College of Environmental Science and Engineering, Hunan University, Changsha, 410082, Hunan, China
3. Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, Hunan, China
2. Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310012, Zhejiang, China - ISSN：1432-0614
文摘
A new bioflocculant was produced by culturing Rhodococcus erythropolis in a cheap medium. When culture pH was 7.0, inoculum size was 2?% (v/v), Na2HPO4 concentration was 0.5?g?L?, and the ratio of sludge/livestock wastewater was 7:1 (v/v), a maximum flocculating rate of 87.6?% could be achieved. Among 13 different kinds of pretreatments for sludge, the optimal one was the thermal-alkaline pretreatment. Different from a bioflocculant produced in a standard medium, this bioflocculant was effective over a wide pH range from 2 to 12 with flocculating rates higher than 98?%. Approximately, 1.6?g?L? of crude bioflocculant could be harvested using cold ethanol for extraction. This bioflocculant showed color removal rates up to 80?% when applied to direct and disperse dye solutions, but only 23.0?% for reactive dye solutions. Infrared spectrum showed that the bioflocculant contained functional groups such as –OH, –NH2, and –CONH2. Components in the bioflocculant consisted of 91.2?% of polysaccharides, 7.6?% of proteins, and 1.2?% of DNA. When the bioflocculant and copper sulfate (CuSO4) were used together for decolorization in actual dye wastewater, the optimum decolorization conditions were specified by the response surface methodology as pH?11, bioflocculant dosage of 40?mg/L, and CuSO4 80?mg/L, under which a decolorization rate of 93.9?% could be reached.