Inhibition of sarco(endo)plasmic reticulum Ca2+-ATPase differentially regulates contractile function in cardiac myocytes from normotensive and spontaneously hypertensive rats
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- 作者：Shi-Yan Li (1)
Kish L. Golden (2)
Yang Jiang (2)
Guei-Jane Wang (3)
Jamie R. Privratsky (1)
Xiaochun Zhang (1)
Anna R. Eason (1)
Bruce Culver (1)
Jun Ren (1) - 关键词：Hypertension ; contractile function ; myocytes ; cell isolation/culture ; calcium
- 刊名：Cell Biochemistry and Biophysics
- 出版年：2005
- 出版时间：February 2005
- 年：2005
- 卷：42
- 期：1
- 页码：1-12
- 全文大小：314KB
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Kish L. Golden (2)
Yang Jiang (2)
Guei-Jane Wang (3)
Jamie R. Privratsky (1)
Xiaochun Zhang (1)
Anna R. Eason (1)
Bruce Culver (1)
Jun Ren (1)
1. Division of Pharmaceutical Sciences and Center for Cardiovascular Research and Alternative Medicine, University of Wyoming College of Health Sciences, 82071-3375, Laramie, WY
2. Department of Physiology, and Internal Medicine, Wayne State University, 48201, Detroit, MI
3. National Research Institute of Chinese Medicine, Taipei, Taiwan, Republic of China
文摘
Hypertension leads to impaired contractile function. This study examined the impact of inhibition of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) by thapsigargin or cyclopiazonic acid (CPA) on cardiac contractile function in ventricular myocytes from Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). Mechanical properties were examined including peak shortening (PS), time-to-PS (TPS), time-to-90% relengthening (TR90), and maximal velocity of shortening/relengthening (±dL/dt). Intracellular Ca2+ transients were evaluated as fura-2 fluorescent intensity (FFI), excitation-induced change in FFI (ΔFFI=peak-basal), and fluorescence decay rate (τ). Expression of Ca2+ regulatory proteins SERCA2a, Na+?Ca2+ exchanger (NCX), and phospholamban (PLB) were assessed by reverse transcriptase polymerase chain reaction and Western blot. SHR rats exhibited elevated blood pressure. SHR myocytes displayed decreased PS±dL/dt, peak FFI, and ΔFFI; shortened TPS; prolonged τ with normal TR90; and basal FFI compared with WKY myocytes. Inhibition of SERCA with thapsigargin (5μM) or CPA (10 μM) significantly depressed PS±dL/dt, baseline FFI, and ΔFFI, and prolonged TPS, TR90, and τ in WKY myocytes. However, SHR myocytes were relatively insensitive to thapsigargin or CPA with only TPS and TR90 prolonged. Both mRNA and protein expressions of NCX and PLB were significantly enhanced, whereas SERCA2a protein abundance was reduced in SHR rats compared with the WKY group. Our data suggest that inhibition of SERCA function differentially affected cardiac contractile function in ventricular myocytes from normotensive and hypertensive rats possibly through reduced SERCA2a, elevated PLB, and NCX expression under hypertension.