Identification of gonadal soma-derived factor involvement in Monopterus albus (protogynous rice field eel) sex change

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• 作者：Yefei Zhu ; Chunlei Wang ; Xiaowu Chen ; Guijun Guan
• 刊名：Molecular Biology Reports
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：43
• 期：7
• 页码：629-637
• 全文大小：10,015 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978
• 卷排序：43

文摘

We studied molecular events and potential mechanisms underlying the process of female-to-male sex transformation in the rice field eel (Monopterus albus), a protogynous hermaphrodite fish in which the gonad is initially a female ovary and transforms into male testes. We cloned and identified a novel gonadal soma derived factor (GSDF), which encodes a member of the transforming growth factor-beta superfamily. gsdf expression was measured in gonads of female, intersex and male with reverse transcription-PCR and gsdf’s role in sex transformation was studied with qPCR, histological analysis and dual-color in situ hybridization assays and compared to other sex-related genes. gsdf was correlated to Sertoli cell differentiation, indicating involvement in testicular differentiation and sex transformation from female to male in this species. A unique expression pattern reveals a potential role of gsdf essential for the sex transformation of rice field eels.KeywordsGSDFSex transformationSex differentiation