Metabolic engineering of Bacillus subtilis for redistributing the carbon flux to 2,3-butanediol by manipulating NADH levels
详细信息 查看全文
- 作者：Taowei Yang ; Zhiming Rao ; Guiyuan Hu ; Xian Zhang ; Mei Liu…
- 关键词：2 ; 3 ; Butanediol ; Manipulating NADH levels ; Disruption ; Bacillus subtilis
- 刊名：Biotechnology for Biofuels
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：8
- 期：1
- 全文大小：1,867 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Biotechnology
Plant Breeding/Biotechnology
Renewable and Green Energy
Environmental Engineering/Biotechnology - 出版者：BioMed Central
- ISSN：1754-6834
文摘
Background Acetoin reductase (Acr) catalyzes the conversion of acetoin to 2,3-butanediol (2,3-BD) with concomitant oxidation of NADH to NAD+. Therefore, intracellular 2,3-BD production is likely governed by the quantities of rate-limiting factor(s) Acr and/or NADH. Previously, we showed that a high level of Acr was beneficial for 2,3-BD accumulation.