Isolation and characterisation of an HpSHP gene from Hosta plantaginea

详细信息    查看全文

• 作者：Guodong Rao (12)
  Ying Wang (12)
  Dandan Zhang (12)
  Di Liu (12)
  Fenglan Li (12)
  Hai Lu (12) luhai1974@yahoo.com
• 关键词：MADS box gene – ; SHATTERPROOF (SHP) – ; Hosta plantaginea Aschers – ; Gynoecium
• 刊名：Molecular Biology Reports
• 出版年：2012
• 出版时间：June 2012
• 年：2012
• 卷：39
• 期：6
• 页码：6887-6894
• 全文大小：2.8 MB
• 参考文献：1. Skipper M, Johansen LB, Pedersen KB, Frederiksen S, Johansen BB (2006) Cloning and transcription analysis of an AGAMOUS- and SEEDSTICK ortholog in the orchid Dendrobium thyrsiflorum (Reichb. f). Gene 366:266–274
  2. Honma T, Goto K (2001) Complexes of MADS-box proteins are sufficient to convert leaves into floral organs. Nature 409:525–529
  3. Kramer EM, Jaramillo MA, Di Stilio VS (2004) Patterns of gene duplication and functional evolution during the diversification of the AGAMOUS subfamily of MADS box genes in angiosperms. Genetics 166:1011–1023
  4. Causier B, Castillo R, Zhou J, Ingram R, Xue Y, Schwarz-Sommer Z, Davies B (2005) Evolution in action: following function in duplicated floral homeotic genes. Curr Biol 15:1508–1512
  5. Pinyopich A, Ditta GS, Savidge B, Liljegren SJ, Baumann E, Wisman E, Yanofsky MF (2003) Assessing the redundancy of MADS-box genes during carpel and ovule development. Nature 424:85–88
  6. Ren J, Sun L, Wang CL, Zhao SL, Leng P (2011) Expression analysis of the cDNA for magnesium chelatase H subunit (CHLH) during sweet cherryfruit ripening and under stress conditions. Plant Growth Regul 63:301–307
  7. Song H, Liu D, Chen X, Ying ZH, Zhang B, Li FL, Lu H (2010) Change of season-specific telomere lengths in Ginkgo biloba L. Mol Biol Rep 37:819–824
  8. Yang Y, Fanning L, Jack T (2003) The K domain mediates heterodimerization of the Arabidopsis floral organ identity proteins, APETALA3 and PISTILLATA. Plant J 33:47–59
  9. Flanagan CA, Hu Y, Ma H (1996) Specific expression of the AGL1 MADS-box gene suggests regulatory functions in Arabidopsis gynoecium and ovule development. Plant J 10:343–353
  10. Alvarez J, Smyth DR (1999) CRABS CLAW and SPATULA, two Arabidopsis genes that control carpel development in parallel with AGAMOUS. Development 126:2377–2386
  11. Favaro R, Pinyopich A, Battaglia R, Kooiker M, Borghi L, Ditta G, Yanofsky MF, Kater MM, Colombo L (2003) MADS-box protein complexes control carpel and ovule development in Arabidopsis. Plant Cell 15:2603–2611
• 作者单位：1. College of Life Sciences and Biotechnology, Beijing Forestry University, No.35, Beijing Haidian district, Qinghua east road, Beijing, 100083 People鈥檚 Republic of China2. Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing, 100083 People鈥檚 Republic of China
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978

文摘

Based on genetic and molecular analyses, the ABC model has been proposed to explain the genetic control of floral development. C-class MADS-box genes play crucial roles in Arabidopsis thaliana development by regulating the organ identities of stamens and gynoecium. The present research reports for the first time the cloning of an HpSHP gene from Hosta plantaginea (Lam.) Aschers. Phylogenetic analysis shows that HpSHP is a member of the C-class MADS-box genes that is closely related to C-lineage SHP homologues from monocot species. Semi-quantitative and real-time polymerase chain reaction analyses show that HpSHP expression is stamen and gynoecium specific. HpSHP also has spatial and temporal expression patterns in the reproductive organs of H. plantaginea. A functional analysis is carried out in Arabidopsis by overexpression of HpSHP. Homeotic transformations of sepals into carpelloid organs, bent ovaries, and prematurely shattering fruits are observed in 35S::HpSHP transgenic plants. All these results show that HpSHP plays a crucial role in gynoecium development.