Citrate exudation induced by aluminum is independent of plasma membrane H+-ATPase activity and coupled with potassium efflux from cluster roots of phosphorus-deficient white lupin
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- 作者：Houqing Zeng (1) (2)
Xumeng Feng (1)
Baolan Wang (3)
Yiyong Zhu (1)
Qirong Shen (1)
Guohua Xu (1) - 关键词：Al tolerance ; Citrate exudation ; Plasma membrane H+ ; ATPase ; K+ efflux ; Cluster root ; White lupin (Lupinus albus)
- 刊名：Plant and Soil
- 出版年：2013
- 出版时间：2 - May 2013
- 年：2013
- 卷：366
- 期：1
- 页码：389-400
- 全文大小：458KB
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Xumeng Feng (1)
Baolan Wang (3)
Yiyong Zhu (1)
Qirong Shen (1)
Guohua Xu (1)
1. Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Ministry of Agriculture, College of Resource and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China
2. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, People’s Republic of China
3. State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences, Beijing, 100093, People’s Republic of China - ISSN：1573-5036
文摘
Aims Exudation of organic acid anions is one of the mechanisms responsible for aluminum (Al) tolerance. The plasma membrane (PM) H+-ATPase is involved in the exudation of organic acid anions. However, the relationship between organic acid exudation and PM?H+-ATPase under Al toxicity remains unclear. This study aims to investigate the correlation among Al-induced citrate exudation, PM?H+-ATPase activity and counterions for citrate release from cluster roots of phosphorus-deficient (?P) white lupin. Methods Al and various pharmacological agents were applied to incubate the cluster roots of P-deficient white lupin; the citrate exudation rate, PM?H+-ATPase activity and ion exudation of cluster roots were examined. Results Citrate exudation from cluster roots of P-deficient white lupin was induced by 50?μM Al treatment within 1.5?h, but no extra increase was found when the duration of Al treatment increased. The PM?H+-ATPase activity of cluster roots was insensitive to Al treatment, irrespective of Al concentration and duration of Al treatment. Al treatment increased K+ efflux but not H+ efflux from cluster roots. After application of pharmacological agents to P-deficient cluster roots under Al treatment or not, vanadate decreased H+ efflux and increased K+ efflux, but had no inhibitory effect on citrate exudation; fusicoccin increased H+ efflux and citrate exudation, but decreased K+ efflux; tetraethylammonium (TEA) chloride, a K+-channel inhibitor, inhibited K+ efflux and increased H+ efflux. Conclusions These results indicate that citrate exudation induced by combined treatment with P-deficiency and Al is independent of PM?H+-ATPase activity, and is coupled with K+ efflux, which may compensate H+ efflux for keeping the charge balance for Al-induced citrate exudation from cluster roots of P-deficient white lupin.