Survivability of parthenogenetic embryos following in vivo transfer in naturally synchronized Capra hircus

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• 作者：Ravi Ranjan (1) (5)
  Rakesh Kumar Singh (1)
  Thirupathi Yasotha (1)
  Manish Kumar (1)
  Kuldeep Kumar (1)
  Renu Singh (1)
  Monzamul Houque (2)
  Vijay Prakash Mourya (4)
  Gyanendra Singh (3)
  Mihir Sarkar (3)
  Bikash Chandra Das (1)
  Sadhan Bag (1)
  
• 关键词：Caprine ; Parthenogenetic embryo ; Embryo transfer ; Developmental potency ; Ultrasonography
• 刊名：In Vitro Cellular & Developmental Biology - Animal
• 出版年：2013
• 出版时间：August 2013
• 年：2013
• 卷：49
• 期：7
• 页码：486-491
• 全文大小：263KB
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• 作者单位：Ravi Ranjan (1) (5)
  Rakesh Kumar Singh (1)
  Thirupathi Yasotha (1)
  Manish Kumar (1)
  Kuldeep Kumar (1)
  Renu Singh (1)
  Monzamul Houque (2)
  Vijay Prakash Mourya (4)
  Gyanendra Singh (3)
  Mihir Sarkar (3)
  Bikash Chandra Das (1)
  Sadhan Bag (1)
  
  1. Reproductive Physiology and Embryo Transfer Technology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
  5. Physiology, Reproduction and Shelter Management Division, Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, India
  2. Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
  4. Climatology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
  3. Nuclear Research Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
  

文摘

The present study was conducted to see the in vivo developmental potency of caprine parthenogenetic embryos generated in a modified way. The good quality caprine oocytes were matured in presence of cytochalasin B (CCB) and then activated by 7% ethanol followed by 2?mM 6-dimethyl amino purine (6-DMAP) and embryo development was recorded. Early stage parthenogenetic embryos (two to four cells) were surgically transferred in recipients (10). The pregnancy diagnosis was done by nonreturn to oestrus, ultrasonography (USG), and progesterone estimation. The levels of progesterone were above normal values (1?ng/ml) of pregnancy and fall below the level of pregnancy just before retuned to oestrus. Progesterone profile revealed that out of ten recipients (G1–G10), four goats (G1, G2, G3, and G5) returned to oestrus after 43?±-.29 (Mean?±?SE) d of embryo transfer and six goats (G4, G6, G7, G8, G9, and G10) did not return to cycle even after 70?d of embryo transfer. In three recipients (G4, G5, and G6), the USG on day?40 revealed that there was fluid filled uterine body with solid fetus-like structure. These might be dead fetus and had started resorption. The progesterone profile also corroborated the assumption of pregnancy in these animals. Authors believe that this may be the first report on in vivo diploid parthenogenetic embryo development in caprine species.