High zinc ion supplementation of more than 30 μM can increase monoclonal antibody production in recombinant Chinese hamster ovary DG44 cell culture

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• 作者：Bong Gyun Kim ; Hong Woo Park
• 关键词：Chemically defined medium ; Chinese hamster ovary cells ; Monoclonal antibody production ; Suspension culture ; Zinc ion
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：100
• 期：5
• 页码：2163-2170
• 全文大小：790 KB
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• 作者单位：Bong Gyun Kim (1)
  Hong Woo Park (1)
  
  1. Department of Chemical Engineering, Hanyang University, Seoul, 133-791, South Korea
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

Effects of high ZnSO₄·7H₂O supplementation on cell growth and monoclonal antibody (mAb) production in chemically defined suspension cultures of recombinant Chinese hamster ovary (rCHO) DG44 cells were examined. The supplementation of ZnSO₄·7H₂O up to 120 μM gradually increased specific mAb production rate of rCHO DG44 cells in the early growth phase (0–4 days of culture). The ZnSO₄·7H₂O concentration for enhancing mAb production without any cytotoxic effects on cell growth was 30–60 μM. In addition of 60 μM ZnSO₄·7H₂O to in-house protein-free medium and in-house chemically defined medium, mAb production was increased 2.0-fold and 6.5-fold, respectively. Moreover, addition of ZnSO₄·7H₂O to three kinds of commercial chemically defined media yielded a greater than 1.2-fold enhancement of mAb production. These data indicate that simple supplementation of a relatively high zinc ion concentration to cell culture media without significant changes of rCHO DG44 cell culture process can be useful for achieving high production of mAb.