Stimulation of HaCaT keratinocyte and rat mesenchymal stromal cell proliferation by femtosecond laser pulses

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• 作者：A. N. Velikanov ; F. E. Gostev ; E. A. Suprunenko ; I. V. Shelaev…
• 关键词：laser pulses ; photobiomodulation ; reactive oxygen species ; mechanobiology ; stimulation of proliferation ; MSC ; HaCaT keratinocytes
• 刊名：Cell and Tissue Biology
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：9
• 期：6
• 页码：441-446
• 全文大小：723 KB
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• 作者单位：A. N. Velikanov (1)
  F. E. Gostev (2)
  E. A. Suprunenko (1)
  I. V. Shelaev (2)
  V. I. Yusupov (3)
  V. A. Nadtochenko (2)
  
  1. Biology Faculty, Moscow State University, Moscow, 119991, Russia
  2. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, 119991, Russia
  3. Institute of Laser and Information Technologies, Russian Academy of Sciences, Moscow, Troitsk, 140700, Russia
  
• 刊物主题：Cell Biology;
• 出版者：Springer US
• ISSN：1990-5203

文摘

The proliferative activity of HaCaT keratinocytes and rat mesenchymal stromal cells (MSCs) in rats subjected to femtosecond laser pulses has been studied. The growth medium were exposed to laser pulses with a wavelength of 590 nm and duration of 30 fs. Proliferative activity was assessed from the cell number 1 day after the exposure. Cell proliferation in both cell cultures was dose-dependent within the range of 6-299 J/cm2. Increased proliferative activity was observed at the lowest doses (32-4% for HaCaT cells and 19% for MSCs). High doses did not affect the cell growth. No increase in the reactive oxygen species was registered in the culture medium using physicochemical analysis. Femtosecond laser pulses generated acoustic oscillations from 0.5 to 6.0 kHz in the culture medium. It is proposed that the increase in the cell proliferative activity may be caused by the mechanical effects of acoustic waves produced in the environment by optical breakdown in the focus of the laser radiation. Keywords laser pulses photobiomodulation reactive oxygen species mechanobiology stimulation of proliferation MSC HaCaT keratinocytes