Computational study of culture conditions and nutrient supply in a hollow membrane sheet bioreactor for large-scale bone tissue engineering

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• 作者：Ramin Khademi (1)
  Davod Mohebbi-Kalhori (1)
  Afra Hadjizadeh (2) (3)
  
• 关键词：Hollow membrane sheet ; Bioreactor ; Scaffold ; 3D mathematical model ; Bone tissue
• 刊名：Journal of Artificial Organs
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：17
• 期：1
• 页码：69-80
• 全文大小：3,722 KB
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• 作者单位：Ramin Khademi (1)
  Davod Mohebbi-Kalhori (1)
  Afra Hadjizadeh (2) (3)
  
  1. Chemical Engineering Department, University of Sistan and Baluchestan, P.O. Box 98164-161, Zahedan, Iran
  2. Chemical Engineering Department, 脡cole Polytechnique de Montr茅al, 2900 Boulevard Edouard-Montpetit, Montr茅al, QC, H3T 1J4, Canada
  3. Faculty of Biomedical Engineering, Center of Excellence on Biomaterials, AmirKabir University of Technology, Tehran, Iran
  
• ISSN：1619-0904

文摘

Successful bone tissue culture in a large implant is still a challenge. We have previously developed a porous hollow membrane sheet (HMSh) for tissue engineering applications (Afra Hadjizadeh and Davod Mohebbi-Kalhori, J Biomed. Mater. Res. Part A [2]). This study aims to investigate culture conditions and nutrient supply in a bioreactor made of HMSh. For this purpose, hydrodynamic and mass transport behavior in the newly proposed hollow membrane sheet bioreactor including a lumen region and porous membrane (scaffold) for supporting and feeding cells with a grooved section for accommodating gel-cell matrix was numerically studied. A finite element method was used for solving the governing equations in both homogenous and porous media. Furthermore, the cell resistance and waste production have been included in a 3D mathematical model. The influences of different bioreactor design parameters and the scaffold properties which determine the HMSh bioreactor performance and various operating conditions were discussed in detail. The obtained results illustrated that the novel scaffold can be employed in the large-scale applications in bone tissue engineering.