Computational modeling of adherent cell growth in a hollow-fiber membrane bioreactor for large-scale 3-D bone tissue engineering

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• 作者：Davod Mohebbi-Kalhori (16) d.mohebbi@usherbrooke.ca
  Amin Behzadmehr (27)
  Charles J. Doillon (34)
  Afra Hadjizadeh (5) Afra.Hajizadeh@polymtl.ca
• 关键词：Nutrient ; dependent cell growth – ; Hollow ; fiber bioreactor – ; 3 ; D bone tissue – ; Adherent cells – ; Bone marrow ; derived cells
• 刊名：Journal of Artificial Organs
• 出版年：2012
• 出版时间：September 2012
• 年：2012
• 卷：15
• 期：3
• 页码：250-265
• 全文大小：935.2 KB
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• 作者单位：1. Department of Chemical Engineering-Biotechnology, Universit茅 de Sherbrooke, 2500, Boulevard de l鈥橴niversit茅, Sherbrooke, QC J1K 2R1, Canada2. Department of Mechanical Engineering, Universit茅 de Sherbrooke, 2500, Boulevard de l鈥橴niversit茅, Sherbrooke, QC J1K 2R1, Canada3. CHUL鈥檚 Research Center, 2705 Boulevard Laurier, Quebec City, QC G1V 4G2, Canada4. Department of Surgery, Faculty of Medicine, Universit茅 Laval, Quebec City, QC G1K 7P4, Canada5. Department of Chemical Engineering, Ecole Polytechnique of Montreal, C.P. 6079, succ. Centre-ville, Montreal, QC H3C 3A7, Canada6. Department of Chemical Engineering, Faculty of Engineering, University of Sistan and Baluchestan, P.O. Box 98164-161, Zahedan, Iran7. Department of Mechanical Engineering, University of Sistan and Baluchestan, Zahedan, Iran
• ISSN：1619-0904

文摘

The use of hollow-fiber membrane bioreactors (HFMBs) has been proposed for three-dimensional bone tissue growth at the clinical scale. However, to achieve an efficient HFMB design, the relationship between cell growth and environmental conditions must be determined. Therefore, in this work, a dynamic double-porous media model was developed to determine nutrient-dependent cell growth for bone tissue formation in a HFMB. The whole hollow-fiber scaffold within the bioreactor was treated as a porous domain in this model. The domain consisted of two interpenetrating porous regions, including a porous lumen region available for fluid flow and a porous extracapillary space filled with a collagen gel that contained adherent cells for promoting long-term growth into tissue-like mass. The governing equations were solved numerically and the model was validated using previously published experimental results. The contributions of several bioreactor design and process parameters to the performance of the bioreactor were studied. The results demonstrated that the process and design parameters of the HFMB significantly affect nutrient transport and thus cell behavior over a long period of culture. The approach presented here can be applied to any cell type and used to develop tissue engineering hollow-fiber scaffolds.