Identification of the Babesia-responsive leucine-rich repeat domain-containing protein from the hard tick Haemaphysalis longicornis

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• 作者：Hiroki Maeda ; Koshi Kurisu ; Takeshi Miyata ; Kodai Kusakisako…
• 关键词：Leucine ; rich repeat ; Tick ; Babesia ; Recombinant ; Parasite
• 刊名：Parasitology Research
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：114
• 期：5
• 页码：1793-1802
• 全文大小：829 KB
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• 作者单位：Hiroki Maeda (1) (2)
  Koshi Kurisu (1)
  Takeshi Miyata (3)
  Kodai Kusakisako (1) (2)
  Remil Linggatong Galay (1) (2)
  Talactac Melbourne Rio (1) (2)
  Masami Mochizuki (1) (2)
  Kozo Fujisaki (4)
  Tetsuya Tanaka (1) (2)
  
  1. Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima, 890-0065, Japan
  2. Department of Pathological and Preventive Veterinary Science, United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi, 753-8515, Japan
  3. Division of Molecular Functions of Food, Department of Biochemistry and Biotechnology, Kagoshima University, Korimoto, Kagoshima, 890-0065, Japan
  4. National Agricultural and Food Research Organization, Tsukuba, Ibaraki, 305-0856, Japan
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

Haemaphysalis longicornis is a tick known for transmitting Babesia parasites, including Babesia gibsoni, in East Asian countries. The vector tick must have strategies to control Babesia parasites, while Babesia parasites are also considered to establish an evasive mechanism from the tick’s innate immunity. Due to this mutual tolerance, H. longicornis is considered to be a vector of Babesia parasites. Recent studies have shown the important roles of leucine-rich repeat (LRR) domain-containing proteins in innate immunity in many living organisms. Some LRR domain-containing proteins were identified in ticks; however, their functions are still unknown. In this study, a novel LRR domain-containing protein was identified from H. longicornis (HlLRR). HlLRR contains two LRR domains, and the expression levels of mRNA and proteins were upregulated during blood feeding, particularly in the salivary glands and midgut. In addition, recombinant HlLRR (rHlLRR) demonstrated growth inhibition activity against B. gibsoni in vitro without a hemolytic effect at any concentration used. Moreover, the diameters of Babesia merozoites treated with rHlLRR were significantly larger than those of the control group. These results strongly indicate the key roles of HlLRR in the tick’s innate immunity against Babesia parasites. Furthermore, HlLRR might be a potential alternative drug to treat babesiosis.