Cloning and molecular analysis of voraxin-α gene of Rhipicephalus (Boophilus) microplus

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• 作者：Binod Kumar ; Srikanta Ghosh
• 关键词：Voraxin ; α ; Anti ; tick vaccine ; Rhipicephalus (Boophilus) microplus
• 刊名：Journal of Parasitic Diseases
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：40
• 期：1
• 页码：184-188
• 全文大小：636 KB
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• 作者单位：Binod Kumar (1) (2)
  Srikanta Ghosh (1)
  
  1. Entomology Laboratory, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, 243122, India
  2. Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, 362001, India
  
• 刊物主题：Infectious Diseases; Health Promotion and Disease Prevention; Medicine/Public Health, general;
• 出版者：Springer India
• ISSN：0975-0703

文摘

To identify suitable targets for development of cross-protective tick vaccine, in silico analysis was attempted and male tick derived molecule, voraxin-α was targeted. The voraxin-α homologue of Rhipicephalus (Boophilus) microplus was cloned, sequenced and analyzed employing standard methods. The deduced amino acids sequence analysis of the 419 bp cloned voraxin-α gene of R. (B.) microplus indicated very high (94.6 %) similarity with voraxin-α of the R. appendiculatus and moderate to low identity with Amblyomma hebraeum, Dermacentor silvarum and Haemaphysalis longicornis. The results suggest that recombinant voraxin-α might be a good candidate as cross-protective anti-tick vaccine.