文摘
Purpose Excessive nitrate (NO<sub>3sub> ?/sup>) accumulation in greenhouse vegetable soils often results in nitrogen (N) loss through leaching or gaseous emission as a result of denitrification. Dissimilatory NO<sub>3sub> ?/sup> reduction to ammonium (NH<sub>4sub> +) (DNRA) as affected by organic amendments in a greenhouse vegetable soil was studied. Materials and methods Soil incubations were carried out following the amendment of rice straw (RS), Chinese milk vetch (CMV), and a control (CK) without organic amendment using two 15?N isotope methods in the laboratory. Results and discussion Gross DNRA rates were 15.2-6.2 and 0.21-.06?mg?N?kg??day? when estimated by the recovery of 15NO<sub>3sub> ?/sup> in NH<sub>4sub> + and organic N (ON) pools (M1) and by mean residence time (MRT) of NH<sub>4sub> + (M2), respectively. Microbial NO<sub>3sub> ?/sup> assimilation might occur due to high NO<sub>3sub> ?/sup> content in the tested soil (1.27?g?N?kg?), and thus, M1 probably overestimated gross DNRA rates. Gross DNRA rates estimated by M2 were higher in RS and CMV treatments than those in CK and were significantly (p-lt;-.05) correlated with soil dissolved organic carbon (DOC) content and the ratio of DOC to NO<sub>3sub> ?/sup>–N. Conclusions Therefore, the DNRA rate was promoted by organic amendments attributed to the enhancement of soil DOC to NO<sub>3sub> ?/sup>–N ratio. Further studies on DNRA in more soil types under different conditions are required in order to better understand the potential contribution of DNRA to N transformation and conservation in vegetable soils.