QTL mapping and analysis of the embryo and maternal plant for three limiting amino acids in rapeseed meal

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• 作者：Jianfeng Xu ; Yan Long ; Jianguo Wu ; Haiming Xu…
• 关键词：Genetic main effect ; Limiting amino acid ; Quantitative trait ; Quantitative trait locus (QTL) ; QTL?×?environment interaction effect ; Rapeseed meal
• 刊名：European Food Research and Technology
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：240
• 期：1
• 页码：147-158
• 全文大小：1,089 KB
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  6. Chen GL, Wu JG, Shi CH (2011) Dynamic genetic effects on threonine content in rapeseed ( / Brassica napus L.) meal at different developmental stages. Czech J Genet Plant Breed 47(3):101-13
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• 作者单位：Jianfeng Xu (1)
  Yan Long (2)
  Jianguo Wu (3)
  Haiming Xu (1)
  Juan Wen (1)
  Jinling Meng (2)
  Chunhai Shi (1)
  
  1. Department of Agronomy, Zhejiang University, Hangzhou, 310058, China
  2. College of Plant Science and Technology, Huazhong Agriculture University, Wuhan, 430070, China
  3. Department of Horticulture, Zhejiang A&F University, Lin’an, 311300, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Analytical Chemistry
  Biotechnology
  Agriculture
  Forestry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1438-2385

文摘

Rapeseed meal is a rich vegetable protein source because of its balanced amino acid content. Lysine, threonine and methionine are three limiting essential amino acids for livestock and poultry on whose inheritance there is little information available especially at molecular level across different environments. In present study, quantitative trait locus (QTL) identification and analysis for these three important amino acids in rapeseed meal were conducted using two backcross populations derived from 202 double haploid lines of an elite hybrid cross “Tapidor?×?Ningyou7 (TN)-and their parents. A newly developed QTL model and related mapping software were used to analyze the genetic main effects and QTL?×?environment (QE) interaction effects for QTLs located in the embryo and maternal genomes, respectively. The results revealed 17 QTLs associated with lysine, threonine and methionine contents in rapeseed meal, 10 of which were in the A sub-genome and the rest in C sub-genome of Brassica napus L., showing significant embryo and maternal genetic effects. Among these QTLs, two were also found to have significant QE interaction effects. In addition, some QTLs were located at the same position or linkage group, such as three QTLs (qLysC-4-2, qThrC-4-2 and qMetC-4-2) in A4 linkage group and two (qThrC-7-3 and qMetC-7-3) in A7 linkage group were co-localized, and three (qLysC-1-1, qThrC-1-1 and qMetC-1-1) were distributed in the same A1 linkage group. qLysC-1-1, qThrC-12-5 and qMetC-7-3 were the major QTLs, which could explain 25.4, 36.7 and 16.4?% of their phenotypic variations, respectively. The genetic main effects and the QE interaction effects of QTLs from embryo and maternal genomes were important for these limiting essential amino acids. The results above might be helpful for marker-assisted selection in rapeseed breeding and cloning of functional genes relevant to lysine, threonine and methionine contents.