Physiological and transcriptional responses of contrasting alfalfa (Medicago sativa L.) varieties to salt stress

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• 作者：Wenli Quan ; Xun Liu ; Haiqing Wang…
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：126
• 期：1
• 页码：105-115
• 全文大小：1,877 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044
• 卷排序：126

文摘

Alfalfa (Medicago sativa L.) acts as a most important legume forage crop and is widely cultivated in various environments. Salt stress is one of the major abiotic stresses in cultivation of alfalfa worldwide. Development of alfalfa cultivars for adaptable to salt environments can provide sustainable solutions. In the present study, we selected two varieties with contrasting salt tolerance—211609 and Xinjiang Daye—from 14 alfalfa varieties. Under salt stress condition, 211609 showed higher leaf water content, less severe cell membrane damage (Electrolyte leakage) and lower accumulation of reactive oxygen species than Xinjiang Daye which exhibited lower GSH content and less antioxidant enzyme activities. In addition, significantly higher expression levels of NHX1, ZFG, CBF4 and HSP23 genes were found in 211609 than those in Xinjiang Daye upon exposure to salt stress. Collectively, these results proved that 211609 showed higher tolerance to salt stress than Xinjiang Daye through regulation of physiological and transcriptional pathways. It could play an important role in breeding program of alfalfa varieties with improved stress tolerance in future.KeywordsAlfalfaSalt stressPhysiological mechanismsStress responsive genesTranscriptional expression