Differential distribution of IL28B.rs12979860 single-nucleotide polymorphism among Egyptian healthcare workers with and without a hepatitis C virus-specific cellular immune response

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• 作者：Sayed F. Abdelwahab ; Zainab Zakaria ; Maha Sobhy ; Shaimaa Hamdy…
• 刊名：Archives of Virology
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：160
• 期：7
• 页码：1741-1750
• 全文大小：521 KB
• 参考文献：1.Abdel-Aziz F, Habib M, Mohamed MK, Abdel-Hamid M, Gamil F, Madkour S, Mikhail NN, Thomas D, Fix AD, Strickland GT, Anwar W, Sallam I (2000) Hepatitis C virus (HCV) infection in a community in the Nile Delta: population description and HCV prevalence. Hepatology 32:111-15PubMed View Article
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• 作者单位：Sayed F. Abdelwahab (1) (2) (3)
  Zainab Zakaria (2)
  Maha Sobhy (2)
  Shaimaa Hamdy (2)
  Mohamed A. Mahmoud (4)
  Nabiel Mikhail (5)
  Walaa R. Allam (2)
  Eman Rewisha (4)
  Imam Waked (4)
  
  1. Department of Microbiology and Immunology, Faculty of Medicine, Minia University, Minia, 61511, Egypt
  2. The Holding Company for Biological Products and Vaccines (VACSERA), 51 Wizaret El-Zeraa St., Agouza, Giza, 22311, Egypt
  3. Department of Microbiology, Taif Faculty of Pharmacy, Al-Haweiah, PO Box 888, Taif, 21974, Kingdom of Saudi Arabia
  4. Department of Hepatology, National Liver Institute, Menoufiya University, Menoufiya, 32511, Egypt
  5. South Egypt Cancer Institute, Asyut, 71526, Egypt
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798

文摘

The CC genotype of the interleukin (IL)-28B.rs12979860 gene has been associated with spontaneous hepatitis C virus (HCV) clearance and treatment response. The distribution and correlation of an IL28B.rs12979860 single-nucleotide polymorphism (SNP) with HCV-specific cell-mediated immune (CMI) responses among Egyptian healthcare workers (HCWs) is not known. We determined this relationship in 402 HCWs who serve a patient cohort with ~85?% HCV prevalence. We enrolled 402 HCWs in four groups: group 1 (n?=?258), seronegative aviremic subjects; group 2 (n?=?25), seronegative viremic subjects; group 3 (n?=?41), subjects with spontaneously resolved HCV infection; and group 4 (n?=?78), chronic HCV patients. All subjects were tested for an HCV-specific CMI response using an ex-vivo interferon-gamma (IFNγ) ELISpot assay with nine HCV genotype-4a overlapping 15-mer peptide pools corresponding to all of the HCV proteins. All subjects were tested for IL28B.rs12979860 SNP by real-time PCR. An HCV-specific CMI was demonstrated in ~27?% of the seronegative aviremic HCWs (group 1), suggesting clearance of infection after low-level exposure to HCV. The frequency of IL28B.rs12979860 C allele homozygosity in the four groups was 49?%, 48?%, 49?%, and 23?%, while that of the T allele was 14?%, 16?%, 12 and 19?%, respectively, suggesting differential distributions among subjects with different HCV status. As reported, IL28B.rs12979860 predicted the outcome of HCV infection (p?<?0.05), but we did not find any relationship between the IL28B genotypes and the outcome of HCV-specific CMI responses in the four groups (p?>?0.05). The data show differential IL28B.rs12979860 genotype distribution among Egyptian HCWs with different HCV status and could not predict the outcome of HCV-specific CMI responses.