Physiologic roles of soluble pyridine nucleotide transhydrogenase inEscherichia coli as determined by homologous recombination
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- 作者：Hanjun Zhao (1) (2)
Peng Wang (1) (2)
Enqi Huang (1) (2)
Yadong Ge (1) (2)
Guoping Zhu (1) (2) - 关键词：soluble transhydrogenase ; homologous recombination ; growth rate ; isocitrate dehydrogenase ; NADH dehydrogenase II ; real ; time PCR
- 刊名：Annals of Microbiology
- 出版年：2008
- 出版时间：June 2008
- 年：2008
- 卷：58
- 期：2
- 页码：275-280
- 全文大小：166KB
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Peng Wang (1) (2)
Enqi Huang (1) (2)
Yadong Ge (1) (2)
Guoping Zhu (1) (2)
1. The Key Laboratory of Molecular Evolution, Anhui Normal University, 1 Beijing Road, 241000, Wuhu, Anhui, P.R. China
2. Institute of Molecular Biology and Biotechnology, Anhui Normal University, 1 Beijing Road, 241000, Wuhu, Anhui, P.R. China - ISSN：1869-2044
文摘
The soluble transhydrogenase is an energy-independent flavoprotein and important in cofactor regenerating system. In order to understand its physiologic roles, the recombinant strain with the deletion of soluble transhydrogenase gene (ΔudhA) inEscherichia coli was constructed using homologous recombination. Then the different genetic back-grounds containing eithericd NADP oricd NAD, which encodes NADP-dependent isocitrate dehydrogenase (IDH) or engineered NAD-dependent IDH, were transduced into ΔudhA, creating two strains (icd NADP/ΔudhA, icd NAD/ΔudhA). During growth on acetate,icd NADP/ΔudhA grew poorly and its growth rate was remarkably reduced by 75% as compared with the wild type. However,icd NAD/ΔudhA showed significantly better growth thanicd NADP/ΔudhA. Its growth rate was about 3.7 fold oficd NADP/ΔudhA, which was equivalent to the wild type. These results indicated that UdhA is an essential NADH resource for acetate-grownE. coli and is a dominant factor for bacteria to adapt to the stress environment. Furthermore, when UdhA was absence,icd NAD/ΔudhA displayed about 1.5 fold increase in the IDH activity after switching the carbon source from glucose to acetate. And RT-PCR showed that the expression of NADH dehydrogenase II (NDH-2) inicd NAD/ΔudhA was remarkably up-regulated by about 2.8 fold as compared withicd NADP/ΔudhA. The increase of IDH activity and NDH-2 expression can be explained by the reducing excess NADPH production and restoring higher levels of NADH generation in cells.