Effect of sonication on a novel designed peptide

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• 作者：Liping Ruan (1)
  Hanlin Luo (2)
  Hangyu Zhang (2)
  Zhihua Xing (3)
  
• 关键词：peptide ; nanofiber ; atomic force microscopy ; circular dichroism ; rheology
• 刊名：Journal of Wuhan University of Technology--Materials Science Edition
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：28
• 期：3
• 页码：622-626
• 全文大小：324KB
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• 作者单位：Liping Ruan (1)
  Hanlin Luo (2)
  Hangyu Zhang (2)
  Zhihua Xing (3)
  
  1. School of Chemical Engineering, Sichuan University, Chengdu, 610065, China
  2. West China Hospital Laboratory of Nanomedicine and West China Hospital Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu, 610041, China
  3. Laboratory of Ethnopharmacology, Institute for Nanobiomedical Technology and Membrane Biology, Regenerative Medicine Research Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, China
  
• ISSN：1993-0437

文摘

The reassembly of a half-sequence ionic self-complementarity peptide CH3CO-Pro-Ser-Phe-Cys-Phe-Lys-Phe-Glu-Pro-NH2 was reported, which could self-assemble into stable nanofibers and formed hydrogel consisting of >99% water. In this study, the nanofiber scaffold was sonicated by an ultrasonic cell disruptor. The effects of sonication were detected by circular dichroism (CD), atomic force microscopy (AFM), and rheology. AFM image illustrated that the sonicated fragments could quickly reassemble into nanofibers, while the morphology was distinguishable from the original one. CD spectrum revealed that the conversion occurred mainly between regular β-strand structure and distorted β-strand structure. Rheological analyses showed that the storage modulus (G- of the peptide solution at the 7th day after sonication decreased by nearly 40% compared with the value of the solution before sonication. Finally, a plausible conversion model was proposed to interpret the reassembly process.