Responses of pelargonium (Pelargonium × hortorum L.H. Bailey) to long-term salinity stress induced by treatment with different NaCl doses

详细信息    查看全文

• 作者：Włodzimierz Breś ; Hanna Bandurska ; Agnieszka Kupska…
• 关键词：Pelargonium ; Salinity ; Chlorophyll fluorescence ; Chloroplast pigments ; Proline ; Anthocyanins
• 刊名：Acta Physiologiae Plantarum
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：38
• 期：1
• 全文大小：594 KB
• 参考文献：Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Env Exp Bot 59:206–216CrossRef
  Ashraf M, Harris PJC (2004) Potential biochemical indicators of salinity tolerance in plants. Plant Sci 166:1–3CrossRef
  Banu NA, Hoque A, Watanabe-Sugimoto M, Matsouka K, Nakamura Y, Shimoishi Y, Murata Y (2009) Proline and glycinebetaine induce antioxidant defense gene expression and suppress cell death in cultured tobacco cells under salt stress. J Plant Physiol 166:146–156CrossRef PubMed
  Bates LS, Waldren RP, Teare JD (1973) Rapid determination of proline for water stress studies. Plant Soil 39:205–207CrossRef
  Breś W, Kozłowska A, Kupska A (2014) The salinity of soils located along the selected streets of Poznan. Current trends in the horticultural plants cultivation. In: National scientific conference, Lublin-Susiec, 12–13 June 2014, p 40 (in Polish)
  Carillo P, Annunziata MG, Pontecorvo G, Fuggi A, Woodrow P (2011) Salinity stress and salt tolerance. In: Shanker AK, Venkateswarlu B (eds) Abiotic stress in plants—mechanisms and adaptations. InTech, Croatia, pp 21–38
  Cassaniti C, Romano D, Flowers TJ (2012) The response of ornamental plants to saline irrigation water. In: Garcia-Garizabal I (ed) Irrigation-water management, pollution and alternative strategies. InTech, Croatia, pp 131–139
  Chakraborty K, Sairam RK, Bhattacharya RC (2012) Salinity-induced expression of pyrrolline-5-carboxylate synthetase determine salinity tolerance in Brassica spp. Acta Physiol Plant 34:1935–1941CrossRef
  Chalker-Scott L (1999) Environmental significance of anthocyanin in plant stress responses. Photochem Photobiol 70:1–17CrossRef
  Chazen O, Neumann PM (1994) Hydraulic signals from the roots and rapid cell wall hardening in growing maize leaves, are primary responses to PEG induced water deficit. Plant Physiol 104:1385–1392PubMedCentral PubMed
  Cramer GR, Bowman DC (1991) Kinetics of maize leaf elongation 1. Indirect yield threshold limit short term steady stead elongation rates after exposure to salinity. J Exp Bot 42:1417–1426CrossRef
  Cunningham MA, Snyder E, Yonkin D, Ross M, Elsen T (2008) Accumulation of deicing salts in soils in urban environment. Urban Ecosyst 11:17–31CrossRef
  De Carvalho K, De Campo MKF, Domingues DS, Pereira LFP, Vieira LGE (2013) The accumulation of endogenous proline induces changes in gene expression of several antioxidant enzymes in leaves of transgenic Swingle citrumelo. Mol Biol Rep 40:3269–3279CrossRef PubMed
  Devecchi M, Remotti D (2004) Effect of salts on ornamental ground covers for green urban areas. Acta Hortic 643:153–156CrossRef
  Dkhil BB, Denden M (2012) Effect of salt stress on growth, anthocyanins, membrane permeability and chlorophyll fluorescence of okra (Abelmoschus esculentus L.) seedlings. Am J Plant Physiol 7:174–183CrossRef
  Eryilmaz F (2006) The relationships between salt stress and anthocyanin content in higher plants. Biotechnol Biotechnol Eq 20:47–52CrossRef
  Filippou P, Bouchagier P, Skotti E, Fotopoulos V (2014) Proline and reactive oxygen/nitrogen species metabolism is involved in the tolerant response of the invasive plant species Ailanthus altissima to drought and salinity. Env Exp Bot 97:1–10CrossRef
  Gaxiola RA, Li J, Undurraga S, Dang LM, Allen GJ, Alper SL, Fink GR (2001) Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump. Proc Natl Acad Sci USA 981:11444–11449CrossRef
  Gururani MA, Upadhaya ChP, Strasser RJ, Yu JW, Park SW (2013) Evaluation of abiotic stress tolerance in transgenic potato plants with reduced expression of PII manganese stabilizing protein. Plant Sci 198:7–16CrossRef PubMed
  Hayat S, Hayat Q, Alymeni MN, Wani AS, Pichtel J, Ahmed A (2012) Role of proline under changing environments. Plant Signal Behav 7:1–11CrossRef
  Hiscox JC, Israelstam GF (1979) A method for the extraction of chlorophyll from tissue without maceration. Can J Bot 57:1332–1334CrossRef
  Hossain MA, Fujita M (2010) Evidence for a role of exogenous glycinebetaine and proline in antioxidant defense and methylglyoxal detoxification systems in mung bean seedlings under salt stress. Physiol Mol Biol Plant 16:19–29CrossRef
  Hughes NM, Reinhardt K, Field TS, Gerardi AR, Smith WK (2010) Association between winter anthocyanin production and drought stress in angiosperm evergreen species. J Exp Bot 61:1699–1709PubMedCentral CrossRef PubMed
  Iqbal N, Umar S, Khan NA, Iqbal M, Khan R (2014) A new perspective of phytohormones in salinity tolerance: regulation of proline metabolism. Env Exp Bot 100:34–42CrossRef
  Iqbal N, Khan R, Nazir F, Asgher M, Per TS, Khan NA (2015) Selenium and sulfur influence ethylene formation and alleviate cadmium-induced oxidative stress by improving proline and glutathione production in wheat. J Plant Physiol 173:9–18CrossRef
  Jaleel CA, Sankar B, Sridharan R, Panneerselvam R (2008) Soil salinity alters growth, chlorophyll content and secondary metabolite accumulation in Catharanthus roseus. Turk J Biol 32:79–83
  Jamil M, Rehman S, Lee KJ, Kim JM, Kim HS, Rhae S (2007) Salinity reduced growth PS2 photochemistry and chlorophyll content in radish. Scientia Agricola 64:1111–1118CrossRef
  Karla YP (1998) Handbook of reference methods for plant analysis. CRC Press, Taylor & Francis Group, Boca Raton, p 287
  Khan MIR, Iqbal N, Masood A, Khan NA (2012) Variation in salt tolerance of wheat cultivars: role of glycinebetaine and ethylene. Pedosphere 22:746–754CrossRef
  Khavari-Nejad RA, Bujar M, Attaran E (2008) Evaluation of anthocyanin contents under salinity (NaCl) stress in Bellis perennis L. In: Khan MA, Weber DJ (eds) Ecophysiology of high salinity tolerant plants. Springer, pp 124–134
  Kotuby-Amacher J, Koenig R, Kitchen B (2000) Salinity and plant tolerance. Electronic publishing. All Archived Publications. Paper 43. http://​digitalcommons.​usu.​edu/​extension_​histall/​43
  Kubala S, Wojtyla Ł, Quinet M, Lechowska K, Lutts S, Garnczarska M (2015) Enhanced expression of proline synthesis gene P5CSA in relation to seed osmopriming improvement of Brassica napus germination under salinity stress. J Plant Physiol 183:1–12CrossRef PubMed
  Lu C, Qiu N, Lu Q, Wang B, Kuang T (2002) Does salt stress lead to increased susceptibility of photosystem II to photoinhibition and changes in photosynthetic pigment composition in halophyte Suaeda salsa grown outdoors? Plant Sci 163:1063–1068CrossRef
  Mahajan S, Tuteja N (2005) Cold, salinity and drought stresses: an overview. Archiv Biochem Biophys 444:139–158CrossRef
  Maxwell K, Johnson GN (2000) Chlorophyll fluorescence: a practical guide. J Exp Bot 51:659–668CrossRef PubMed
  Meloni DA, Oliva MA, Martinez CA, Cambria J (2003) Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Env Exp Bot 49:69–76CrossRef
  Miyamoto S (2008) Salt Tolerance of landscape plants common to the southwest. Texas A&M Univ Sys TR 316:1–37
  Moradi F, Ismail AM (2007) Responses of photosynthesis, chlorophyll fluorescence and ROS-scavenging systems to salt stress during seedling and reproductive stages in rice. Ann Bot 99:1161–1173PubMedCentral CrossRef PubMed
  Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681CrossRef PubMed
  Nandy P, Das S, Ghose M, Spooner-Hart R (2007) Effects of salinity on photosynthesis, leaf anatomy, ion accumulation and photosynthetic nitrogen use efficiency in five Indian mangroves. Wetlands Ecol Manag 15:347–357CrossRef
  Neumann PM (1993) Rapid and reversible modification of extension capacity of cell walls in elongating maize leaf tissue responding to root addition and removal of NaCl. Plant Cell Env 16:1107–1114CrossRef
  Nguyen ML, Kim G-B, Hyun S-H, Lee S-Y, Lee Ah-Y, Choi H-K, Choi H-K, Nam Y-W (2013) Physiological and metabolomic analysis of a knockout mutant suggests a critical role of MtP5CS gene in osmotic stress tolerance of Medicago truncatula. Euphytica 193:101–120CrossRef
  Rachoski M, Gazquez A, Calzadilla P, Bezus R, Rodriguez A, Ruiz O, Menendez A, Santiago M (2015) Chlorophyll fluorescence and lipid peroxidation changes in rice somaclonal lines subjected to salt stress. Acta Physiol Plant 37:117. doi:10.​1007/​s11738-015-1865-0 CrossRef
  Roy SJ, Negrão S, Tester M (2014) Salt resistant crop plants. Curr Op Biotech 26:115–124CrossRef
  Schultz HR, Matthews MA (1993) Growth, osmotic adjustment, and cell wall mechanics of expanding grape leaves during water deficits. Crop Sci 33:287–294CrossRef
  Shannon MC, Grieve CM (1999) Tolerance of vegetable crops to salinity. Sci Hortic 78:5–38CrossRef
  Singh AK, Dubey RS (1995) Changes in chlorophyll a and b contents and activities of photosystems 1 and 2 in rice seedlings induced by NaCl. Photosynthetica 31:489–499
  Stępień P, Kłobus G (2006) Water relations and photosynthesis in Cucumis sativus L. leaves under salt stress. Biol Plant 50:610–616CrossRef
  Surekha C, Kumari KN, Aruna LV, Suneetha G, Arundhati A, Kishor PK (2014) Expression of the Vigna aconitifolia P5CSF129A gene in transgenic pigeon pea enhances proline accumulation and salt tolerance. Plant Cell Tiss Org Cult 116:27–36CrossRef
  Szabados L, Savouré A (2010) Proline: multifunctional amino acid. Trends Plant Sci 15:89–97CrossRef PubMed
  Tavakkoli E, Rengasamy P, McDonald GK (2010) High concentration of Na+ and Cl− ions in soil solution have simultaneous detrimental effects on growth of faba bean under salinity stress. J Exp Bot 61:4449–4459PubMedCentral CrossRef PubMed
  Tavakkoli E, Fatehi F, Coventry S, Rengasamy P, McDonald KG (2011) Additive effects of Na+ and Cl− ions on barley growth under salinity stress. J Exp Bot 62:2189–2203PubMedCentral CrossRef PubMed
  Türkan YI, Demiral T (2009) Recent developments in understanding salinity tolerance. Env Exp Bot 67:2–9CrossRef
  Veatch-Blohm ME, Malinowski M, Keefer D (2012) Leaf water status, osmotic adjustment and carbon assimilation in colored calla lilies in response to saline irrigation. Sci Hortic 144:65–73CrossRef
  Verslues PE, Sharma S (2010) Proline metabolism and its implications for plant-environment interaction. Arabidopsis Book 8:e0140. doi:10.​1199/​tab.​0140 PubMedCentral CrossRef PubMed
  Verslues PE, Agarwal M, Katiyar-Agarwal S, Zhu J, Zhu J-K (2006) Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. Plant J 45:5235–5239CrossRef
  Villarino GH, Mattson NS (2011) Assessing tolerance to sodium chloride salinity in fourteen floriculture species. HortTechnol 21:539–545
  Wahid A, Ghazanfar A (2006) Possible involvement of some secondary metabolites in salt tolerance of sugarcane. J Plant Physiol 163:723–730CrossRef PubMed
  Wallender WW, Tanji KT (2012) Agricultural salinity assessment and management, 2nd edn. American Society of Civil Engineers (eds), p 1094. doi:10.​1061/​9780784411698.​fm
  Wang H, Arakawa O, Motomura Y (2000) Influence of maturity and bagging on relationship between anthocyanin accumulation and phenylalanine ammonia-lyase (pal) activity in ‘Jonathan’ apples. Postharvest Biol Technol 19:123–128CrossRef
  Weatherly PE (1950) Studies in water relation of cotton plants. The measurement of water deficits in leaves. New Phytol 49:81–97CrossRef
  Weinhold F, Scharpf H-C (1997) Tolerance of ornamental plants to salt, sodium and chloride in potting substrates containing compost made of separately collected organic residues. Acta Hort 450:221–228CrossRef
  Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144:307–313CrossRef
  Wilkaniec B, Breś W, Frużyńska-Jóźwiak D, Borowiak-Sobkowiak B, Wilkaniec A (2012) The assessment of chemical properties of soil, the chemical composition of leaves and the occurrence of diseases on Acer platanoides and Tilia cordata in selected sites of urban greenery in Poznań. Phytopathol 65:19–28
  Winkel-Shirley B (2002) Biosynthesis of flavonoids and effects of stress. Curr Op Plant Biol 5:218–223CrossRef
  Xiong L, Zhu J-K (2002) Salt tolerance. Arabidopsis Book 1:e0048. doi:10.​1199/​tab.​0048 PubMedCentral CrossRef PubMed
  Yazici I, Türkan I, Sekman AH, Demiral T (2007) Salinity tolerance of purslane (Portulaca oleracea L.) is achieved by enhanced antioxidative system, lower level of lipid peroxidation and proline accumulation. Env Exp Bot 61:49–57CrossRef
  
• 作者单位：Włodzimierz Breś (1)
  Hanna Bandurska (2)
  Agnieszka Kupska (1)
  Justyna Niedziela (2)
  Barbara Frąszczak (3)
  
  1. Department of Plant Nutrition, Poznań University of Life Sciences, Zgorzelecka 4, 60-198, Poznań, Poland
  2. Department of Plant Physiology, Poznań University of Life Sciences, Wołyńska 35, 60-637, Poznań, Poland
  3. Department of Vegetable Crops, Poznań University of Life Sciences, Dąbrowskiego 159, 60-594, Poznań, Poland
  
• 刊物主题：Plant Physiology; Plant Genetics & Genomics; Plant Biochemistry; Plant Pathology; Plant Anatomy/Development; Agriculture;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-1664

文摘

The aim of this research was to study the physiological and biochemical responses of pelargonium growing in saline substrate. Salt stress caused an increase of sodium and chlorine, and decrease potassium ions concentrations in pelargonium leaves depending on their level in peat substrate. About 4–16-fold increase of sodium, 4–6-fold increase of chlorine were found in leaves of plants growing in substrate treated with the lowest (452 mg dm−3) and highest (2992 mg dm−3) NaCl doses, respectively. The concentration of potassium ions decreased by 20 to 27 % in leaves of plants growing in substrate supplemented with NaCl doses from 1976 to 2992 mg dm−3, respectively. However, lower doses of sodium chloride did not affect the content of potassium ions in pelargonium leaves. The increasing salinity of substrate after the addition of 960–2992 mg NaCl dm−3 caused the reduction of plant fresh matter in the range of 25–65 %, plant height 10–37 %, and leaf area 15–55 %. There were no changes in relative water content (RWC) and no signs of damage in the form of necrotic spots for any of the used salt concentrations. The content of chlorophyll pigments decreased proportionally to salt concentration, but the content of carotenoids did not change. Maximum photochemical activity of PSII (F _v/F _m) was reduced only in plants growing at the highest dose of NaCl. Proline and anthocyanin levels increased in response to elevated NaCl concentration. At the highest dose of NaCl proline level increased by 30 % while the content of anthocyanins increased about 2.5-fold in relation to the control. It can be assumed that proline and anthocyanins accumulated in pelargonium leaves may be responsible for the amelioration of the adverse effects of salt stress. The results revealed that the investigated pelargonium ‘Survivor Dark Red’ is somewhat tolerant to salinity and it can be cultivated in substrate and soil polluted with NaCl at the level lower than 1976 mg dm−3. Keywords Pelargonium Salinity Chlorophyll fluorescence Chloroplast pigments Proline Anthocyanins