Up-converted fluorescence from photosynthetic light-harvesting complexes linearly dependent on excitation intensity

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• 作者：Kristjan Leiger ; Arvi Freiberg
• 关键词：Bacteriochlorophyll聽a ; Fluorescence ; Delayed fluorescence ; Two ; photon excitation ; Two ; step excitation ; Triplet鈥搕riplet annihilation
• 刊名：Photosynthesis Research
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：127
• 期：1
• 页码：77-87
• 全文大小：1,009 KB
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• 作者单位：Kristjan Leiger (1)
  Arvi Freiberg (1) (2)
  
  1. Institute of Physics, University of Tartu, Ravila 14c, 51011, Tartu, Estonia
  2. Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51014, Tartu, Estonia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5079

文摘

Weak up-converted fluorescence related to bacteriochlorophyll a was recorded from various detergent-isolated and membrane-embedded light-harvesting pigment鈥損rotein complexes as well as from the functional membranes of photosynthetic purple bacteria under continuous-wave infrared laser excitation at 1064 nm, far outside the optically allowed singlet absorption bands of the chromophore. The fluorescence increases linearly with the excitation power, distinguishing it from the previously observed two-photon excited fluorescence upon femtosecond pulse excitation. Possible mechanisms of this excitation are discussed. Keywords Bacteriochlorophyll a Fluorescence Delayed fluorescence Two-photon excitation Two-step excitation Triplet鈥搕riplet annihilation